Ubiquitin B Antibody (1F5)

Novus Biologicals, part of Bio-Techne | Catalog # H00007314-M01

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Product Datasheet / COA / SDS

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1F5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

UBB (AAH09301, 1 a.a. ~ 76 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG

Localization

Cytoplasmic and Nuclear

Specificity

UBB - ubiquitin B

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Ubiquitin B Antibody (1F5)

Western Blot: Ubiquitin B Antibody (1F5) [H00007314-M01]

Western Blot: Ubiquitin B Antibody (1F5) [H00007314-M01] - UBB expression in MCF-7 ( Cat # L046V1 ).

Immunohistochemistry-Paraffin: Ubiquitin B Antibody (1F5) [H00007314-M01]

Immunohistochemistry-Paraffin: Ubiquitin B Antibody (1F5) [H00007314-M01] - Analysis of monoclonal antibody to UBB on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.

Sandwich ELISA: Ubiquitin B Antibody (1F5) [H00007314-M01] - Detection limit for recombinant GST tagged UBB is approximately 0.03ng/ml as a capture antibody.

Applications for Ubiquitin B Antibody (1F5)

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Ubiquitin+1

This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.

Long Name

frame shift mutant

Alternate Names

FLJ25987, MGC8385, polyubiquitin B, polyubiquitin-B, RPS27A, UBA52, UBC, ubiquitin B

Entrez Gene IDs

7314 (Human)

Gene Symbol

UBB

OMIM

191339 (Human)

UniProt

AAH09301 (Human)

Additional Ubiquitin+1 Products

Product Documents for Ubiquitin B Antibody (1F5)

Product Datasheets

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COA

SDS

Product Specific Notices for Ubiquitin B Antibody (1F5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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