Ubiquitin B Antibody (3C12) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37521

Novus Biologicals, part of Bio-Techne
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NBP2-37521
NBP2-37521-0.025ml
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3C12

Format

BSA Free

Concentration

1 mg/ml

View all Ubiquitin+1 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Ubiquitin B expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ubiquitin B Antibody (3C12) - BSA Free

Western Blot: Ubiquitin B Antibody (3C12)BSA Free [NBP2-37521]

Western Blot: Ubiquitin B Antibody (3C12)BSA Free [NBP2-37521]

Western Blot: Ubiquitin B Antibody (3C12) [NBP2-37521] - Western blot analysis using UBB mouse mAb against NIH/3T3 (1) and Hela (2) cell lysate.
Flow Cytometry: Ubiquitin B Antibody (3C12) - BSA Free [NBP2-37521]

Flow Cytometry: Ubiquitin B Antibody (3C12) - BSA Free [NBP2-37521]

Flow Cytometry: Ubiquitin B Antibody (3C12) [NBP2-37521] - Flow cytometric analysis of Hela cells using UBB mouse mAb (green) and negative control (red).
Western Blot: Ubiquitin B Antibody (3C12)BSA Free [NBP2-37521]

Western Blot: Ubiquitin B Antibody (3C12)BSA Free [NBP2-37521]

Western Blot: Ubiquitin B Antibody (3C12) [NBP2-37521] - Western blot analysis using UBB mAb against human UBB (AA: 1-299) recombinant protein. (Expected MW is 26 kDa)
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Applications for Ubiquitin B Antibody (3C12) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ubiquitin+1

ubiquitin B encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.

Long Name

frame shift mutant

Alternate Names

FLJ25987, MGC8385, polyubiquitin B, polyubiquitin-B, RPS27A, UBA52, UBC, ubiquitin B

Gene Symbol

UBB

Additional Ubiquitin+1 Products

Product Documents for Ubiquitin B Antibody (3C12) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ubiquitin B Antibody (3C12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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