Ubiquitin B Antibody (rUBB/9476)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44622
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG2b Kappa Clone # rUBB/9476
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant fragment of human Ubiquitin protein (around aa 1-119) (Uniprot: P0G47; P0G48; P62979; P62987; P62988). Exact sequence is proprietary.
Localization
Cell membrane, Secreted
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of Ab produced in CHO cell mammalian-based expression system.. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Applications for Ubiquitin B Antibody (rUBB/9476)
Application
Recommended Usage
Immunohistochemistry
1-2ug/ml
Western Blot
2-4ug/ml
Application Notes
Immunohistochemistry: 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Ubiquitin+1
Long Name
frame shift mutant
Alternate Names
FLJ25987, MGC8385, polyubiquitin B, polyubiquitin-B, RPS27A, UBA52, UBC, ubiquitin B
Gene Symbol
UBB
Additional Ubiquitin+1 Products
Product Documents for Ubiquitin B Antibody (rUBB/9476)
Product Specific Notices for Ubiquitin B Antibody (rUBB/9476)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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