Ubiquitin B Antibody (UBB/1748) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08905

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2c Kappa Clone # UBB/1748

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Ubiquitin+1 Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human Ubiquitin B protein (around aa 1-119) (exact sequence is proprietary)

Localization

Cell Surface, Cytoplasmic and Nuclear

Marker

Autophagy Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2c Kappa

Theoretical MW

9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07163).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Ubiquitin B Antibody (UBB/1748) - Azide and BSA Free

Western Blot: Ubiquitin B Antibody (UBB/1748)Azide and BSA Free [NBP3-08905]

Western Blot: Ubiquitin B Antibody (UBB/1748)Azide and BSA Free [NBP3-08905]

Western Blot: Ubiquitin B Antibody (UBB/1748) [NBP3-08905] - Western Blot Analysis of human Raji cell lysate using Ubiquitin B-Monospecific Mouse Monoclonal Antibody (UBB/1748).
Immunohistochemistry-Paraffin: Ubiquitin B Antibody (UBB/1748) - Azide and BSA Free [NBP3-08905]

Immunohistochemistry-Paraffin: Ubiquitin B Antibody (UBB/1748) - Azide and BSA Free [NBP3-08905]

Immunohistochemistry-Paraffin: Ubiquitin B Antibody (UBB/1748) [NBP3-08905] - Formalin-fixed, paraffin-embedded human Brain stained with Ubiquitin B-Monospecific Mouse Monoclonal Antibody (UBB/1748).
Flow Cytometry: Ubiquitin B Antibody (UBB/1748) - Azide and BSA Free [NBP3-08905]

Flow Cytometry: Ubiquitin B Antibody (UBB/1748) - Azide and BSA Free [NBP3-08905]

Flow Cytometry: Ubiquitin B Antibody (UBB/1748) [NBP3-08905] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using Ubiquitin B-Monospecific Mouse Monoclonal Antibody (UBB/1748) followed by goat anti- Mouse IgG-CF488 (Blue); Isotype Control (Red).
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Applications for Ubiquitin B Antibody (UBB/1748) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Ubiquitin+1

ubiquitin B encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.

Long Name

frame shift mutant

Alternate Names

FLJ25987, MGC8385, polyubiquitin B, polyubiquitin-B, RPS27A, UBA52, UBC, ubiquitin B

Gene Symbol

UBB

Additional Ubiquitin+1 Products

Product Documents for Ubiquitin B Antibody (UBB/1748) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ubiquitin B Antibody (UBB/1748) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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