UBR2 Antibody (PCRP-UBR2-1D12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13900

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # PCRP-UBR2-1D12

Concentration

0.2 mg/ml

View all UBR2 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human UBR2 protein (Uniprot: Q8IWV8)

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

200.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14287)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for UBR2 Antibody (PCRP-UBR2-1D12)

Immunocytochemistry/ Immunofluorescence: UBR2 Antibody (PCRP-UBR2-1D12) [NBP3-13900]

Immunocytochemistry/ Immunofluorescence: UBR2 Antibody (PCRP-UBR2-1D12) [NBP3-13900]

Immunocytochemistry/Immunofluorescence: UBR2 Antibody (PCRP-UBR2-1D12) [NBP3-13900] - Flow cytometric analysis of PFA-fixed HeLa cells. UBR2 antibody (PCRP-UBR2-1D12) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Immunohistochemistry-Paraffin: UBR2 Antibody (PCRP-UBR2-1D12) [NBP3-13900]

Immunohistochemistry-Paraffin: UBR2 Antibody (PCRP-UBR2-1D12) [NBP3-13900]

Immunohistochemistry-Paraffin: UBR2 Antibody (PCRP-UBR2-1D12) [NBP3-13900] - Flow cytometric analysis of PFA-fixed HeLa cells. UBR2 antibody (PCRP-UBR2-1D12) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Flow Cytometry: UBR2 Antibody (PCRP-UBR2-1D12) [NBP3-13900]

Flow Cytometry: UBR2 Antibody (PCRP-UBR2-1D12) [NBP3-13900]

Flow Cytometry: UBR2 Antibody (PCRP-UBR2-1D12) [NBP3-13900] - Flow cytometric analysis of PFA-fixed HeLa cells. UBR2 antibody (PCRP-UBR2-1D12) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
View 4 more images

Applications for UBR2 Antibody (PCRP-UBR2-1D12)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: UBR2

Proteolysis by the ubiquitin-proteasome system controls the concentration of many regulatory proteins. The selectivity of ubiquitylation is determined by ubiquitin E3 ligases, which recognize the substrate's destabilization signal, or degron. The E3 ligase UBR2 participates in the N-end rule pathway, which targets proteins bearing an N-terminal degron, or N-degron (Kwon et al., 2003 [PubMed 14585983]).[supplied by OMIM]

Alternate Names

bA49A4.1, C6orf133MGC71112, chromosome 6 open reading frame 133, dJ242G1.1, dJ392M17.3, DKFZp686C08114, E3 ubiquitin-protein ligase UBR2, EC 6.3.2.-, KIAA0349FLJ36357, N-recognin-2, RP3-392M17.3, ubiquitin protein ligase E3 component n-recognin 2, Ubiquitin-protein ligase E3-alpha-2, Ubiquitin-protein ligase E3-alpha-II

Gene Symbol

UBR2

UniProt

Q8IWV8 (Human)

Additional UBR2 Products

Product Documents for UBR2 Antibody (PCRP-UBR2-1D12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UBR2 Antibody (PCRP-UBR2-1D12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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