UBR2 Antibody (PCRP-UBR2-1D12) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14287

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # PCRP-UBR2-1D12

Format

Azide and BSA Free

Concentration

1 mg/ml

View all UBR2 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human UBR2 protein (Uniprot: Q8IWV8)

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

200.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13900).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for UBR2 Antibody (PCRP-UBR2-1D12) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: UBR2 Antibody (PCRP-UBR2-1D12) - Azide and BSA Free [NBP3-14287]

Immunocytochemistry/ Immunofluorescence: UBR2 Antibody (PCRP-UBR2-1D12) - Azide and BSA Free [NBP3-14287]

Immunocytochemistry/Immunofluorescence: UBR2 Antibody (PCRP-UBR2-1D12) - Azide and BSA Free [NBP3-14287] - Flow cytometric analysis of PFA-fixed HeLa cells. UBR2 antibody (PCRP-UBR2-1D12) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Immunohistochemistry-Paraffin: UBR2 Antibody (PCRP-UBR2-1D12) - Azide and BSA Free [NBP3-14287]

Immunohistochemistry-Paraffin: UBR2 Antibody (PCRP-UBR2-1D12) - Azide and BSA Free [NBP3-14287]

Immunohistochemistry-Paraffin: UBR2 Antibody (PCRP-UBR2-1D12) - Azide and BSA Free [NBP3-14287] - Flow cytometric analysis of PFA-fixed HeLa cells. UBR2 antibody (PCRP-UBR2-1D12) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Protein Array: UBR2 Antibody (PCRP-UBR2-1D12)Azide and BSA Free [NBP3-14287]

Protein Array: UBR2 Antibody (PCRP-UBR2-1D12)Azide and BSA Free [NBP3-14287]

Protein Array: UBR2 Antibody (PCRP-UBR2-1D12) - Azide and BSA Free [NBP3-14287] - Analysis of Protein Array containing more than 19,000 full-length human proteins using UBR2 Antibody (PCRP-UBR2-1D12).
View 1 more images

Applications for UBR2 Antibody (PCRP-UBR2-1D12) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Immunoprecipitation

1-2 ug per 100-500 ug protein

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: UBR2

Proteolysis by the ubiquitin-proteasome system controls the concentration of many regulatory proteins. The selectivity of ubiquitylation is determined by ubiquitin E3 ligases, which recognize the substrate's destabilization signal, or degron. The E3 ligase UBR2 participates in the N-end rule pathway, which targets proteins bearing an N-terminal degron, or N-degron (Kwon et al., 2003 [PubMed 14585983]).[supplied by OMIM]

Alternate Names

bA49A4.1, C6orf133MGC71112, chromosome 6 open reading frame 133, dJ242G1.1, dJ392M17.3, DKFZp686C08114, E3 ubiquitin-protein ligase UBR2, EC 6.3.2.-, KIAA0349FLJ36357, N-recognin-2, RP3-392M17.3, ubiquitin protein ligase E3 component n-recognin 2, Ubiquitin-protein ligase E3-alpha-2, Ubiquitin-protein ligase E3-alpha-II

Gene Symbol

UBR2

Additional UBR2 Products

Product Documents for UBR2 Antibody (PCRP-UBR2-1D12) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for UBR2 Antibody (PCRP-UBR2-1D12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.