UBR4 Antibody (2D12)

Novus Biologicals, part of Bio-Techne | Catalog # H00023352-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2D12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all UBR4 Antibodies »

Product Specifications

Immunogen

RBAF600 (NP_065816, 94 a.a. ~ 190 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RNQLQSVAAACKVLIEFSLLRLENPDEACAVSQKHLILLIKGLCTGCSRLDRTEIITFTAMMKSAKLPQTVKTLSDVEDQKELASPVSPELRQKEVQ

Reactivity Notes

Human. Other species not tested.

Specificity

RBAF600 - retinoblastoma-associated factor 600

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for UBR4 Antibody (2D12)

Western Blot: UBR4 Antibody (2D12) [H00023352-M01]

Western Blot: UBR4 Antibody (2D12) [H00023352-M01]

Western Blot: UBR4 Antibody (2D12) [H00023352-M01] - RBAF600 expression in K-562 ( Cat # L009V1 ).
Immunocytochemistry/ Immunofluorescence: UBR4 Antibody (2D12) [H00023352-M01]

Immunocytochemistry/ Immunofluorescence: UBR4 Antibody (2D12) [H00023352-M01]

Immunocytochemistry/Immunofluorescence: UBR4 Antibody (2D12) [H00023352-M01] - Analysis of monoclonal antibody to RBAF600 on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: UBR4 Antibody (2D12) [H00023352-M01] - Detection limit for recombinant GST tagged RBAF600 is approximately 0.1ng/ml as a capture antibody.

Applications for UBR4 Antibody (2D12)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UBR4

p600 is an E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. It recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Together with clathrin, p600 forms meshwork structures involved in membrane morphogenesis and cytoskeletal organization. Additionally p600 regulates integrin-mediated signaling and may play a role in activation of FAK in response to cell-matrix interactions.

Alternate Names

600 kDa retinoblastoma protein-associated factor, EC 6.3.2, EC 6.3.2.-, KIAA0462E3 ubiquitin-protein ligase UBR4, KIAA1307zinc finger, UBR1 type 1, N-recognin-4, p600, RBAF600FLJ41863, Retinoblastoma-associated factor of 600 kDa, ubiquitin protein ligase E3 component n-recognin 4, Zinc finger UBR1-type protein 1, ZUBR1retinoblastoma-associated factor 600-like protein

Entrez Gene IDs

23352 (Human)

Gene Symbol

UBR4

UniProt

NP_065816 (Human)

Additional UBR4 Products

Product Documents for UBR4 Antibody (2D12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UBR4 Antibody (2D12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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