UBR5/EDD Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-71802
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 2749 and 2799 of human E3 Ubiquitin Protein Ligase, HECT domain using the numbering given in entry NP_056986.2 (GeneID 51366).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for UBR5/EDD Antibody
UBR5/EDD Antibody [NBP1-71802] - Detection of human and mouse EDD1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (H; 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (5, 15, and 50 ug for WB) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-EDD1 antibody NBP1-71802 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. EDD1 was less efficiently immunoprecipitated by another rabbit anti-EDD1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Applications for UBR5/EDD Antibody
Application
Recommended Usage
Immunoprecipitation
2 - 5 ug/mg lysate
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: UBR5
Long Name
Ubiquitin Protein Ligase E3 Component N-recognin 5
Alternate Names
DD5, EDD, EDD1, HYD
Gene Symbol
UBR5
UniProt
Additional UBR5 Products
Product Documents for UBR5/EDD Antibody
Product Specific Notices for UBR5/EDD Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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