Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine, Canine, Guinea Pig, Rabbit, Sheep, Zebrafish

Applications

Immunocytochemistry/ Immunofluorescence, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 13C4

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all UCH-L1/PGP9.5 Antibodies »

Product Specifications

Immunogen

UCH-L1/PGP9.5 protein from brain (Uniprot: P09936)

Localization

Cytoplasmic. Endoplasmic Reticulum membrane

Marker

pan-Neuronal Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-29420).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for UCH-L1/PGP9.5 Antibody (13C4) - Azide and BSA Free

Western Blot: UCH-L1/PGP9.5 Antibody (13C4)Azide and BSA Free [NBP2-33186]

Western Blot: UCH-L1/PGP9.5 Antibody (13C4)Azide and BSA Free [NBP2-33186]

Western Blot: UCH-L1/PGP9.5 Antibody (13C4) - Azide and BSA Free [NBP2-33186] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (13C4)
Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody (13C4) - Azide and BSA Free [NBP2-33186]

Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody (13C4) - Azide and BSA Free [NBP2-33186]

Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody (13C4) - Azide and BSA Free [NBP2-33186] - Immunofluorescence Analysis of T98G cells labeling Pgp9.5 with UCH-L1/PGP9.5 Antibody (13C4) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red)
Simple Western: UCH-L1/PGP9.5 Antibody (13C4)Azide and BSA Free [NBP2-33186]

Simple Western: UCH-L1/PGP9.5 Antibody (13C4)Azide and BSA Free [NBP2-33186]

Simple Western: UCH-L1/PGP9.5 Antibody (13C4) - Azide and BSA Free [NBP2-33186] - Simple Western lane view shows a specific band for PGP9.5 / UCHL-1 in 0.2 mg/ml of h. Cerebellum (left) and IMR-32 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
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Applications for UCH-L1/PGP9.5 Antibody (13C4) - Azide and BSA Free

Application
Recommended Usage

Western Blot

0.5-1ug/ml
Application Notes
This antibody has been shown to pair with clone 31A3 (NB600-1160) in ELISA (Gaunitz et al, 2009).

In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: UCH-L1/PGP9.5

Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) is a soluble cytoplasmic protein found in neurons and in cells of the diffuse neuroendocrine system, and their respective tumors. Therefore, UCH-L1 has been widely used as a peripheral nerve fiber marker. UCHL-1 functions as a tissue-specific ubiquitin carboxyl terminal hydrolase isoenzyme involved in the processing of both ubiquitin precursors and ubiquitinated proteins.

UCH L1 is down-regulated in brains from Parkinson disease and Alzheimer disease patients, and certian site specific mutations in the UCHL1 gene can either increase or decrese the risk of Parkinson's and/or Alzheimer's neurodegenerative diseases.

Human UCHL 1 and the closely related UCHL3 protein have one of the most complicated knot structures ever discovered, with five knot crossings. This knot structure is expected to help the protein resist degradation in the proteasome.

Long Name

Ubiquitin C-terminal Hydrolase L1

Alternate Names

PARK5, PGP9.5, UCHL1

Gene Symbol

UCHL1

Additional UCH-L1/PGP9.5 Products

Product Documents for UCH-L1/PGP9.5 Antibody (13C4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for UCH-L1/PGP9.5 Antibody (13C4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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