Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Canine, Guinea Pig, Rabbit, Sheep, Zebrafish

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Simple Western, Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 13C4

Concentration

0.2 mg/ml

View all UCH-L1/PGP9.5 Antibodies »

Product Specifications

Immunogen

UCH-L1/PGP9.5 protein from brain (Uniprot: P09936)

Localization

Cytoplasmic. Endoplasmic Reticulum membrane

Marker

pan-Neuronal Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33186)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for UCH-L1/PGP9.5 Antibody (13C4)

Western Blot: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420]

Western Blot: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420]

Western Blot: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (13C4)
Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420]

Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420]

Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420] - Immunofluorescence Analysis of T98G cells labeling Pgp9.5 with UCH-L1/PGP9.5 Antibody (13C4) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red)
Simple Western: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420]

Simple Western: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420]

Simple Western: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420] - Simple Western lane view shows a specific band for PGP9.5 / UCHL-1 in 0.2 mg/ml of h. Cerebellum (left) and IMR-32 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
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Applications for UCH-L1/PGP9.5 Antibody (13C4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Simple Western

10 ug/ml

Western Blot

1-2 ug/ml
Application Notes
This antibody has been shown to pair with clone 31A3 (NB600-1160) in ELISA (Gaunitz et al, 2009).

In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: UCH-L1/PGP9.5

PGP9.5 (protein gene product 9.5), also known as UchL1 (ubiquitin C-terminal hydrolase) and PARK5, is a neuronal biomarker and ubquitin system protein (reviewed in Day and Thompson, 2010). PGP9.5 is a highly conserved protein and has shown that it is expressed in neurons and neuroendocrine cells in vertebrates where it comprises about 5-10% of soluble cytoplasmic proteins. A minor proportion of PGP9.5 in brain is tightly bound to the membrane. PGP9.5 is also expressed in human oocytes and spermatogonia. It is important to note that the use of an antibody as a biomarker in this case does not imply that an antibody defines absolute tissue specificity. Rather, it means that PGP9.5 is expressed in neurons and neuroendrocine cells at significantly higher levels than in other cell types.
A mutation in the UchL1 gene has been found to cause a form of Parkinson's disease, and this discovery has spurred considerable research interest in UchL1 and to its alternative name of PARK5.
Antibody to UchL1 is widely used as an immunohistochemical marker of nerves and neuroendocrine cells.

Long Name

Ubiquitin C-terminal Hydrolase L1

Alternate Names

PARK5, PGP9.5, UCHL1

Gene Symbol

UCHL1

UniProt

P09936 (Human)

Additional UCH-L1/PGP9.5 Products

Product Documents for UCH-L1/PGP9.5 Antibody (13C4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for UCH-L1/PGP9.5 Antibody (13C4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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