UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33130

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine, Canine, Zebrafish

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 31A3

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all UCH-L1/PGP9.5 Antibodies »

Product Specifications

Immunogen

UCH-L1/PGP9.5 protein from brain (Uniprot: P09936)

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 30377377).

Localization

Cytoplasmic, Endoplasmic Reticulum membrane

Marker

pan-Neuronal Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NB600-1160).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free

Western Blot: UCH-L1/PGP9.5 Antibody (31A3)Azide and BSA Free [NBP2-33130]

Western Blot: UCH-L1/PGP9.5 Antibody (31A3)Azide and BSA Free [NBP2-33130]

Western Blot: UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free [NBP2-33130] - analysis of UchL1 in 1) human, 2) mouse and 3) rat brain lysate using UchL1 antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and ECL substrate solution were used for this test.
Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free [NBP2-33130]

Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free [NBP2-33130]

Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free [NBP2-33130] - Immunofluorescence Analysis of T98G cells labeling Pgp9.5 with UCH-L1/PGP9.5 Antibody (31A3) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red)
Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free [NBP2-33130]

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free [NBP2-33130]

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free [NBP2-33130] - Formalin-fixed, paraffin-embedded human brain stained with UchL1 antibody (PGP9.5) (1:500), peroxidase-conjugate and DAB chromogen. Staining seen in cytoplasm, ER and membrane. Fixation in 95% ethanol/5% acetic acid for 2-3 hours prior to paraffin embedd
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Applications for UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free

Application
Recommended Usage

Western Blot

0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: UCH-L1/PGP9.5

Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) is a soluble cytoplasmic protein found in neurons and in cells of the diffuse neuroendocrine system, and their respective tumors. Therefore, UCH-L1 has been widely used as a peripheral nerve fiber marker. UCHL-1 functions as a tissue-specific ubiquitin carboxyl terminal hydrolase isoenzyme involved in the processing of both ubiquitin precursors and ubiquitinated proteins.

UCH L1 is down-regulated in brains from Parkinson disease and Alzheimer disease patients, and certian site specific mutations in the UCHL1 gene can either increase or decrese the risk of Parkinson's and/or Alzheimer's neurodegenerative diseases.

Human UCHL 1 and the closely related UCHL3 protein have one of the most complicated knot structures ever discovered, with five knot crossings. This knot structure is expected to help the protein resist degradation in the proteasome.

Long Name

Ubiquitin C-terminal Hydrolase L1

Alternate Names

PARK5, PGP9.5, UCHL1

Entrez Gene IDs

7345 (Human); 29545 (Rat)

Gene Symbol

UCHL1

UniProt

P09936 (Human)

Additional UCH-L1/PGP9.5 Products

Product Documents for UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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