UCH-L3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86037

Novus Biologicals, part of Bio-Techne
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NBP1-86037
NBP1-86037-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all UCH-L3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MEGQRWLPLEANPEVTNQFLKQLGLHPNWQFVDVYGMDPELLSMVPRPVCAVLLLFPITEKYEVFRTEEEEKIKSQGQDVTSSVYFMKQTISN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UCH-L3 Antibody

Western Blot: UCH-L3 Antibody [NBP1-86037]

Western Blot: UCH-L3 Antibody [NBP1-86037]

Western Blot: UCH-L3 Antibody [NBP1-86037] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: UCH-L3 Antibody [NBP1-86037]

Immunocytochemistry/ Immunofluorescence: UCH-L3 Antibody [NBP1-86037]

Immunocytochemistry/Immunofluorescence: UCH-L3 Antibody [NBP1-86037] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: UCH-L3 Antibody [NBP1-86037]

Immunohistochemistry-Paraffin: UCH-L3 Antibody [NBP1-86037]

Immunohistochemistry-Paraffin: UCH-L3 Antibody [NBP1-86037] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
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Applications for UCH-L3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UCH-L3

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8

Long Name

Ubiquitin C-terminal Hydrolase L3

Alternate Names

Ubiquitin Thioesterase L3, UCHL3

Gene Symbol

UCHL3

Additional UCH-L3 Products

Product Documents for UCH-L3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UCH-L3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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