UCH-L3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98339

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Mouse

Applications

ELISA, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all UCH-L3 Antibodies »

Product Summary for UCH-L3 Antibody

Immunogen

Produced in rabbits immunized with purified, recombinant Mouse UCH-L3 (Uniprot#: Q9JKB1; Glu 2-Ala 230)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for UCH-L3 Antibody

Western Blot: UCH-L3 Antibody [NBP2-98339]

Western Blot: UCH-L3 Antibody [NBP2-98339]

Western Blot: UCH-L3 Antibody [NBP2-98339] - Anti-UCH-L3 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 27 kDa
Immunohistochemistry-Paraffin: UCH-L3 Antibody [NBP2-98339]

Immunohistochemistry-Paraffin: UCH-L3 Antibody [NBP2-98339]

Immunohistochemistry-Paraffin: UCH-L3 Antibody [NBP2-98339] - Immunochemical staining of mouse UCH-L3 in mouse heart with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Immunohistochemistry-Paraffin: UCH-L3 Antibody [NBP2-98339]

Immunohistochemistry-Paraffin: UCH-L3 Antibody [NBP2-98339]

Immunohistochemistry-Paraffin: UCH-L3 Antibody [NBP2-98339] - Immunochemical staining of mouse UCH-L3 in mouse brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
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Applications for UCH-L3 Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UCH-L3

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8

Long Name

Ubiquitin C-terminal Hydrolase L3

Alternate Names

Ubiquitin Thioesterase L3, UCHL3

Gene Symbol

UCHL3

Additional UCH-L3 Products

Product Documents for UCH-L3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UCH-L3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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