UCKL1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98566

Novus Biologicals, part of Bio-Techne
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NBP2-98566-50ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all UCKL1 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human UCKL1 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for UCKL1 Antibody

Western Blot: UCKL1 Antibody [NBP2-98566]

Western Blot: UCKL1 Antibody [NBP2-98566]

Western Blot: UCKL1 Antibody [NBP2-98566] - Anti-UCKL1 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 61 kDa (We are unsure as to the identity of these extra bands).
Immunocytochemistry/ Immunofluorescence: UCKL1 Antibody [NBP2-98566]

Immunocytochemistry/ Immunofluorescence: UCKL1 Antibody [NBP2-98566]

Immunocytochemistry/Immunofluorescence: UCKL1 Antibody [NBP2-98566] - Immunofluorescence staining of UCKL1 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human UCKL1 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
Immunoprecipitation: UCKL1 Antibody [NBP2-98566]

Immunoprecipitation: UCKL1 Antibody [NBP2-98566]

Immunoprecipitation: UCKL1 Antibody [NBP2-98566] - UCKL1 was immunoprecipitated using: Lane A: 0.5 mg HeLa Whole Cell Lysate 4 ul anti-UCKL1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-UCKL1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 61 kDa

Applications for UCKL1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UCKL1

UCKL1 (Uridine-cytidine kinase 1-like 1) belongs to the uridine kinase family. It is part of the ribonucleotide salvage pathway and may contribute to the UTP accumulation needed for blast transformation and proliferation.

Alternate Names

EC 2.7.1.48, UCK1L, UCK1-LIKE, uridine kinase-like 1, uridine-cytidine kinase 1-like 1, uridine-cytidine kinase-like 1, URKL1FLJ20517

Gene Symbol

UCKL1

Additional UCKL1 Products

Product Documents for UCKL1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for UCKL1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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