UFSP2 Antibody (PSH03-57)

Western Blot: UFSP2 Antibody (PSH03-57) [NBP3-33081]

Western Blot: UFSP2 Antibody (PSH03-57) [NBP3-33081] - Western blot analysis of UFSP2 on different lysates with Rabbit anti-UFSP2 antibody (NBP3-33081) at 1/2,000 dilution.

Lane 1: 293T cell lysate
Lane 2: HeLa cell lysate
Lane 3: HepG2 cell lysate
Lane 4: U-87 MG cell lysate
Lane 5: Neuro-2a cell lysate
Lane 6: RAW264.7 cell lysate
Lane 7: PC-12 cell lysate
Lane 8: C6 cell lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 92 kDa
Observed band size: 92 kDa

Exposure time: 1 minute 40 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33081) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: UFSP2 Antibody (PSH03-57) [NBP3-33081]

Immunohistochemistry: UFSP2 Antibody (PSH03-57) [NBP3-33081] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-UFSP2 antibody (NBP3-33081) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33081) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.