ULK1 Antibody (JA58-36)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66765

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-66765
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JA58-36

Concentration

1 mg/ml

View all ULK1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human ULK1 aa 930-979 / 1050. (SwissProt: O75385 Human; SwissProt: O70405 Mouse; SwissProt: D3ZMG0 Rat)

Localization

Cytosol, Preautophagosomal structure.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ULK1 Antibody (JA58-36)

Western Blot: ULK1 Antibody (JA58-36) [NBP2-66765]

Western Blot: ULK1 Antibody (JA58-36) [NBP2-66765]

Western Blot: ULK1 Antibody (JA58-36) [NBP2-66765] - Analysis of ULK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysateLane 2: MCF-7 cell lysate Lane 3: mouse spleen tissue lysate
Immunohistochemistry-Paraffin: ULK1 Antibody (JA58-36) [NBP2-66765]

Immunohistochemistry-Paraffin: ULK1 Antibody (JA58-36) [NBP2-66765]

Immunohistochemistry-Paraffin: ULK1 Antibody (JA58-36) [NBP2-66765] - Analysis of paraffin-embedded rat skeleton muscle tissue using anti-ULK1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: ULK1 Antibody (JA58-36) [NBP2-66765]

Immunohistochemistry-Paraffin: ULK1 Antibody (JA58-36) [NBP2-66765]

Immunohistochemistry-Paraffin: ULK1 Antibody (JA58-36) [NBP2-66765] - Analysis of paraffin-embedded human colon cancer tissue using anti-ULK1 antibody. Counter stained with hematoxylin.
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Applications for ULK1 Antibody (JA58-36)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ULK1

ULK1 belongs to the serine/threonine protein kinase family. It is involved in axon growth and plays an essential role in neurite branching during sensory axon outgrowth. Knockdown of ULK1 results in impaired endocytosis of nerve growth factor (NGF), excessive axon arborization, and severely stunted axon elongation indicating that ULK1 mediates a non clathrin coated endocytosis in sensory growth cones. Knockdown of ULK1 also inhibits the autophagic response. It appears to act as a convergence point for multiple signals that regulate autophagy, and in turn interacts with a large number of autophagy related (Atg) proteins.

Long Name

Serine/threonine-protein kinase ULK1

Alternate Names

ATG1, hATG1, KIAA0722

Gene Symbol

ULK1

Additional ULK1 Products

Product Documents for ULK1 Antibody (JA58-36)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ULK1 Antibody (JA58-36)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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