uPAR Antibody (A6E8-R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33084
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # A6E8-R
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within human uPAR aa 51-250/335. (Uniprot: Q03405)
Localization
Cell membrane, invadopodium membrane; Secreted.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for uPAR Antibody (A6E8-R)
Western Blot: uPAR Antibody (A6E8-R) [NBP3-33084]
Western Blot: uPAR Antibody (A6E8-R) [NBP3-33084] - Western blot analysis of uPAR on different lysates with Mouse anti-uPAR antibody (NBP3-33084) at 1/1,000 dilution.Lane 1: U937 cell lysate
Lane 2: U937 treated with 200 nM PMA for 72 hours cell lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 37 kDa
Observed band size: 37-60 kDa
Exposure time: 15 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33084) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for uPAR Antibody (A6E8-R)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: uPAR
Long Name
Urokinase-type Plasminogen Activator Receptor
Alternate Names
PLAUR
Gene Symbol
PLAUR
Additional uPAR Products
Product Documents for uPAR Antibody (A6E8-R)
Product Specific Notices for uPAR Antibody (A6E8-R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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