UQCRC1 Antibody (OTI1G6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03825

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey, Primate

Cited:

Mouse, Rat

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1G6

Concentration

1 mg/ml

View all UQCRC1 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 46-305 of human UQCRC1 (NP_003356) produced in E.coli.

Reactivity Notes

Use in Rat reported in scientific literature (PMID:33728024). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

52.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UQCRC1 Antibody (OTI1G6)

Western Blot: UQCRC1 Antibody (OTI1G6) [NBP2-03825]

Western Blot: UQCRC1 Antibody (OTI1G6) [NBP2-03825]

Western Blot: UQCRC1 Antibody (OTI1G6) [NBP2-03825] - Effect of BGP-15 treatment on the electron transport chain complex I and III proteins in NRCMs. Western blot analysis of UQCRC1 (NBP2-03825) and Ndufs1 (NBP1-31142) proteins is shown. GAPDH was used as a loading control. Control group: cells without any treatment; BGP-15 group: cells with only 50uM BGP-15 for 0.5 hours; H2O2group: cells with 150uM H2O2for 0.5 hours; H2O2+BGP-15 group: cells with 150uM H2O2and 50uM BGP-15 for 0.5 hours.Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33728024/) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: UQCRC1 Antibody (OTI1G6) [NBP2-03825]

Immunohistochemistry-Paraffin: UQCRC1 Antibody (OTI1G6) [NBP2-03825]

Immunohistochemistry-Paraffin: UQCRC1 Antibody (OTI1G6) [NBP2-03825] - Staining of paraffin-embedded Human prostate tissue using anti-UQCRC1 mouse monoclonal antibody.
Flow Cytometry: UQCRC1 Antibody (OTI1G6) [NBP2-03825]

Flow Cytometry: UQCRC1 Antibody (OTI1G6) [NBP2-03825]

Flow Cytometry: UQCRC1 Antibody (OTI1G6) [NBP2-03825] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-UQCRC1 antibody, and then analyzed by flow cytometry.
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Applications for UQCRC1 Antibody (OTI1G6)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UQCRC1

This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This protein may mediate formation of the complex between cytochromes c and c1

Alternate Names

Complex III subunit 1, Core protein I, cytochrome b-c1 complex subunit 1, mitochondrial, D3S3191, QCR1, ubiquinol-cytochrome c reductase core protein I, Ubiquinol-cytochrome-c reductase complex core protein 1, UQCR1

Entrez Gene IDs

7384 (Human)

Gene Symbol

UQCRC1

Additional UQCRC1 Products

Product Documents for UQCRC1 Antibody (OTI1G6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UQCRC1 Antibody (OTI1G6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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