UQCRC1 Antibody (PSH01-23)

Western Blot: UQCRC1 Antibody (PSH01-23) [NBP3-33078]

Western Blot: UQCRC1 Antibody (PSH01-23) [NBP3-33078] - Western blot analysis of UQCRC1 on different lysates with Rabbit anti-UQCRC1 antibody (NBP3-33078) at 1/5,000 dilution.

Lane 1: HeLa cell lysate (20 ug/Lane)
Lane 2: HepG2 cell lysate (20 ug/Lane)
Lane 3: HCT 116 cell lysate (20 ug/Lane)
Lane 4: 293T cell lysate (20 ug/Lane)
Lane 5: PC-3M cell lysate (20 ug/Lane)
Lane 6: Jurkat cell lysate (20 ug/Lane)
Lane 7: NIH/3T3 cell lysate (20 ug/Lane)
Lane 8: PC-12 cell lysate (20 ug/Lane)
Lane 9: Mouse kidney tissue lysate (40 ug/Lane)
Lane 10: Mouse brain tissue lysate (40 ug/Lane)
Lane 11: Rat kidney tissue lysate (40 ug/Lane)
Lane 12: Rat brain tissue lysate (40 ug/Lane)

Predicted band size: 53 kDa
Observed band size: 45 kDa

Exposure time: 8 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33078) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: UQCRC1 Antibody (PSH01-23) [NBP3-33078]

Immunohistochemistry: UQCRC1 Antibody (PSH01-23) [NBP3-33078] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-UQCRC1 antibody (NBP3-33078) at 1/5,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33078) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry: UQCRC1 Antibody (PSH01-23) [NBP3-33078]

Immunohistochemistry: UQCRC1 Antibody (PSH01-23) [NBP3-33078] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling UQCRC1 with Rabbit anti-UQCRC1 antibody (NBP3-33078) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33078, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).