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UQCRFS1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32367

Novus Biologicals, part of Bio-Techne
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NBP1-32367

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Single Cell Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human UQCRFS1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (88%).

Localization

Mitochondrion inner membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UQCRFS1 Antibody

Western Blot: UQCRFS1 Antibody [NBP1-32367]

Western Blot: UQCRFS1 Antibody [NBP1-32367]

Western Blot: UQCRFS1 Antibody [NBP1-32367] - Raji cell lysate (30 ug) separated by 12% SDS PAGE and probed with antibody at 1:500.
Immunocytochemistry/ Immunofluorescence: UQCRFS1 Antibody [NBP1-32367]

Immunocytochemistry/ Immunofluorescence: UQCRFS1 Antibody [NBP1-32367]

Immunocytochemistry/Immunofluorescence: UQCRFS1 Antibody [NBP1-32367] - H1299 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: UQCRFS1 protein stained by UQCRFS1 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.
Western Blot: UQCRFS1 Antibody [NBP1-32367]

Western Blot: UQCRFS1 Antibody [NBP1-32367]

Western Blot: UQCRFS1 Antibody [NBP1-32367] - Mouse brain cell lysate (50 ug) separated by 12% SDS PAGE and probed with antibody at 1:1000.

Applications for UQCRFS1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:1000

Single Cell Western

100 ug/mL

Western Blot

1:500 - 1:3000
Application Notes
Single Cell Western reported by an internal validation on HeLa cells at a concentration of 100ug/ml

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UQCRFS1

RIP1 is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis Function: The transit peptide of the Rieske protein seems

Alternate Names

Complex III subunit 5, Cytochrome b-c1 complex subunit 5, cytochrome b-c1 complex subunit Rieske, mitochondrial, EC 1.10.2.2, Rieske iron-sulfur protein, RIP1, RIS1, RISPUbiquinol-cytochrome c reductase iron-sulfur subunit, ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1, UQCR5

Gene Symbol

UQCRFS1

Additional UQCRFS1 Products

Product Documents for UQCRFS1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for UQCRFS1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.