Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all USF2 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 296 and 346 of human Upstream Transcription Factor 2 using the numbering given in entry NP_003358.1 (GeneID 7392).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for USF2 Antibody

Immunohistochemistry: USF2 Antibody [NBP1-92650]

Immunohistochemistry: USF2 Antibody [NBP1-92650]

Immunohistochemistry: USF2 Antibody [NBP1-92650] - Samples: FFPE serial sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti- USF2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
Immunoprecipitation: USF2 Antibody [NBP1-92650]

Immunoprecipitation: USF2 Antibody [NBP1-92650]

Immunoprecipitation: USF2 Antibody [NBP1-92650] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-USF2 antibody NBP1-92647 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. USF2 was also immunoprecipitated by rabbit anti-USF2 antibodies NBP1-92649 and NBP1-92650, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Immunoprecipitation: USF2 Antibody [NBP1-92650]

Immunoprecipitation: USF2 Antibody [NBP1-92650]

Immunoprecipitation: USF2 Antibody [NBP1-92650] - Detection of human USF2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-USF2 antibody NBP1-92650 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. USF2 was also immunoprecipitated by rabbit anti-USF2 antibodies NBP1-92649 and NBP1-92647, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Applications for USF2 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: USF2

USF2 encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. Two transcript variants encoding distinct isoforms have been identified for this gene.

Alternate Names

BHLHB12, bHLHb12upstream stimulatory factor 2, Class B basic helix-loop-helix protein 12, FIPc-fos interacting protein, FOS-interacting protein, Major late transcription factor 2, Upstream transcription factor 2, upstream transcription factor 2, c-fos interacting

Gene Symbol

USF2

Additional USF2 Products

Product Documents for USF2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for USF2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.