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USP22 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49644

Novus Biologicals, part of Bio-Techne
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NBP1-49644SS
NBP1-49644

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Gel Super Shift Assays, Immunohistochemistry, Immunohistochemistry-Paraffin, Proximity Ligation Assay, Simple Western, Western Blot

Cited:

Chemotaxis, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

A genomic peptide made to an internal region of the human USP22 protein (within residues 100-205). [Swiss-Prot Q9H3R0]

Localization

Nucleus.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for USP22 Antibody - BSA Free

Western Blot: USP22 AntibodyBSA Free [NBP1-49644]

Western Blot: USP22 AntibodyBSA Free [NBP1-49644]

Western Blot: USP22 Antibody [NBP1-49644] - Western blot analysis of Usp22 in HeLa whole cell extracts.
Western Blot: USP22 AntibodyBSA Free [NBP1-49644]

Western Blot: USP22 AntibodyBSA Free [NBP1-49644]

Western Blot: USP22 Antibody [NBP1-49644] - analysis of USP22 in HeLa whole cell lysate using anti-USP22 antibody. The primary antibody was used at a dilution of 1:500 and incubated overnight at 4C. Image from verified customer review.
Simple Western: USP22 AntibodyBSA Free [NBP1-49644]

Simple Western: USP22 AntibodyBSA Free [NBP1-49644]

Simple Western: USP22 Antibody [NBP1-49644] - Simple Western lane view shows a specific band for USP22 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for USP22 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation

reported in scientific literature (PMID 34155658)

Gel Super Shift Assays

reported in scientific literature

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 24197134)

Proximity Ligation Assay

reported in scientific literature (PMID 33198416)

Simple Western

1:25

Western Blot

2 ug/ml
Application Notes
In Western blot, a band is seen ~59 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 1 review rated 5 using NBP1-49644 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: USP22

USP22 is the component of the histone acetylation complex, SAGA, that acts as a deubiquinating enzyme. It is a cofactor for gene promoters like MYC. Depleting USP22 prevents MYC from controlling the transcription and expression of thousands of other genes. Therefore, it can be postulated that USP22 is the critical mediator of MYC induced oncogenesis.

Long Name

Ubiquitin Specific Protease 22

Alternate Names

Ubiquitin Thioesterase 22, USP3L

Entrez Gene IDs

23326 (Human); 216825 (Mouse)

Gene Symbol

USP22

Additional USP22 Products

Product Documents for USP22 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for USP22 Antibody - BSA Free

Manufactured by Genomic Antibody Technology™. GAT FAQs

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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