Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all USP25 Antibodies »

Product Specifications

Immunogen

USP25 antibody was raised against a 17 amino acid peptide near the center of human USP25. The immunogen is located within amino acids 470 - 520 of USP25. Amino Acid Squence: IPSQTLPSTTEQQGALS

Specificity

USP25 antibody is human and mouse reactive. Multiple isoforms of USP25 are known to exist.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

105 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for USP25 Antibody - BSA Free

Western Blot: USP25 AntibodyBSA Free [NBP2-82051]

Western Blot: USP25 AntibodyBSA Free [NBP2-82051]

Western Blot: USP25 Antibody [NBP2-82051] - Analysis of USP25 in mouse brain tissue lysate with USP25 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry: USP25 Antibody - BSA Free [NBP2-82051]

Immunohistochemistry: USP25 Antibody - BSA Free [NBP2-82051]

Immunohistochemistry: USP25 Antibody [NBP2-82051] - Immunohistochemistry of USP25 in human brain tissue with USP25 antibody at 5 ug/mL.
USP25 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: USP25 Antibody - BSA Free [NBP2-82051] -

Immunocytochemistry/ Immunofluorescence: USP25 Antibody - BSA Free [NBP2-82051] - Immunofluorescence of USP25 in human brain tissue with USP25 antibody at 20 ug/mL.

Applications for USP25 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

5 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: USP25

USP25 is a ubiquitin-specific protease. Ubiquitin (MIM 191339) is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25 (Valero et al., 1999 [PubMed 10644437]).

Long Name

Ubiquitin Specific Protease 25

Alternate Names

Ubiquitin Thiolesterase 25

Gene Symbol

USP25

Additional USP25 Products

Product Documents for USP25 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for USP25 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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