USP33 Antibody (5B5)

Novus Biologicals, part of Bio-Techne | Catalog # H00023032-M01

Novus Biologicals, part of Bio-Techne
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H00023032-M01
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5B5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all USP33 Antibodies »

Product Specifications

Immunogen

USP33 (NP_055832, 327 a.a. ~ 426 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SDRAENENGSRCFSEDNNETTMLIQDDENNSEMSKDWQKEKMCNKINKVNSEGEFDKDRDSISETVDLNNQETVKVQIHSRASEYITDVHSNDLSTPQIL

Reactivity Notes

Human. Other species not tested.

Localization

Cytoplasmic

Specificity

USP33 - ubiquitin specific protease 33

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for USP33 Antibody (5B5)

Western Blot: USP33 Antibody (5B5) [H00023032-M01]

Western Blot: USP33 Antibody (5B5) [H00023032-M01]

Western Blot: USP33 Antibody (5B5) [H00023032-M01] - Analysis of USP33 expression in transfected 293T cell line by USP33 monoclonal antibody (M01), clone 5B5.Lane 1: USP33 transfected lysate(103.3 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: USP33 Antibody (5B5) [H00023032-M01]

Immunocytochemistry/ Immunofluorescence: USP33 Antibody (5B5) [H00023032-M01]

Immunocytochemistry/Immunofluorescence: USP33 Antibody (5B5) [H00023032-M01] - Analysis of monoclonal antibody to USP33 on HeLa cell. Antibody concentration 20 ug/ml.
Immunohistochemistry-Paraffin: USP33 Antibody (5B5) [H00023032-M01]

Immunohistochemistry-Paraffin: USP33 Antibody (5B5) [H00023032-M01]

Immunohistochemistry-Paraffin: USP33 Antibody (5B5) [H00023032-M01] - Analysis of monoclonal antibody to USP33 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.
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Applications for USP33 Antibody (5B5)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: USP33

The ubiquitin dependent protein degradation pathway is essential for the proteolysis of intracellular proteins and peptides. Deubiquitination, is catalyzed by proteases called deubiquitinating enzymes. USP33 is a deubiquitinating enzyme that binds to the von Hippel-Lindau tumor suppressor protein. It also regulates thyroid hormone activation by deubiquitinating type 2 iodothyronine deiodinase.

Long Name

Ubiquitin carboxyl-terminal hydrolase 33

Alternate Names

hVDU1, KIAA1097, VDU1

Entrez Gene IDs

23032 (Human)

Gene Symbol

USP33

UniProt

NP_055832 (Human)

Additional USP33 Products

Product Documents for USP33 Antibody (5B5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for USP33 Antibody (5B5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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