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USP33 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82931

Novus Biologicals, part of Bio-Techne
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NBP1-82931
NBP1-82931-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ESQVDHSTIHSQETKHYLTVNLTTLRVWCYACSKEVFLDRKLGTQPSLPHVRQPHQIQENSVQDFKIPSNTTLKTPLVAVFDDLDIEADEEDELRARGLTGLKNIGNTCYMNAALQALS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for USP33 Antibody

Western Blot: USP33 Antibody [NBP1-82931]

Western Blot: USP33 Antibody [NBP1-82931]

Western Blot: USP33 Antibody [NBP1-82931] - Analysis in human cell line SCLC-21H.
Immunocytochemistry/ Immunofluorescence: USP33 Antibody [NBP1-82931]

Immunocytochemistry/ Immunofluorescence: USP33 Antibody [NBP1-82931]

Immunocytochemistry/Immunofluorescence: USP33 Antibody [NBP1-82931] - Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm & the Golgi apparatus.
Immunohistochemistry-Paraffin: USP33 Antibody [NBP1-82931]

Immunohistochemistry-Paraffin: USP33 Antibody [NBP1-82931]

Immunohistochemistry-Paraffin: USP33 Antibody [NBP1-82931] - Staining of human kidney shows moderate cytoplasmic positivity in tubule cells.

Applications for USP33 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500-1:1000

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: USP33

The ubiquitin dependent protein degradation pathway is essential for the proteolysis of intracellular proteins and peptides. Deubiquitination, is catalyzed by proteases called deubiquitinating enzymes. USP33 is a deubiquitinating enzyme that binds to the von Hippel-Lindau tumor suppressor protein. It also regulates thyroid hormone activation by deubiquitinating type 2 iodothyronine deiodinase.

Long Name

Ubiquitin carboxyl-terminal hydrolase 33

Alternate Names

hVDU1, KIAA1097, VDU1

Gene Symbol

USP33

Additional USP33 Products

Product Documents for USP33 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for USP33 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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