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Key Product Details

Species Reactivity

Validated:

Human, Canine

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI7G3

Concentration

1 mg/ml

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 589-972 of human USP36 (NP_079366) produced in E.coli.

Marker

Nucleolus Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

122.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for USP36 Antibody (OTI7G3)

Western Blot: USP36 Antibody (OTI7G3) [NBP2-03830]

Western Blot: USP36 Antibody (OTI7G3) [NBP2-03830]

Western Blot: USP36 Antibody (OTI7G3) [NBP2-03830] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-USP36 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunocytochemistry/ Immunofluorescence: USP36 Antibody (OTI7G3) [NBP2-03830]

Immunocytochemistry/ Immunofluorescence: USP36 Antibody (OTI7G3) [NBP2-03830]

Immunocytochemistry/Immunofluorescence: USP36 Antibody (OTI7G3) [NBP2-03830] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY USP36.
Immunohistochemistry-Paraffin: USP36 Antibody (OTI7G3) [NBP2-03830]

Immunohistochemistry-Paraffin: USP36 Antibody (OTI7G3) [NBP2-03830]

Immunohistochemistry-Paraffin: USP36 Antibody (OTI7G3) [NBP2-03830] - Staining of paraffin-embedded Human liver tissue using anti-USP36 mouse monoclonal antibody.

Applications for USP36 Antibody (OTI7G3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: USP36

Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinated action of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP36 belongs to a large family of cysteine proteases that function as deubiquitinating enzymes (Quesada et al., 2004 [PubMed 14715245]).[supplied by OMIM].

Long Name

Ubiquitin Specific Protease 36

Alternate Names

DUB1, KIAA1453

Entrez Gene IDs

57602 (Human)

Gene Symbol

USP36

Additional USP36 Products

Product Documents for USP36 Antibody (OTI7G3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for USP36 Antibody (OTI7G3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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