Key Product Details

Species Reactivity

Validated:

Human, Canine

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI7G3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all USP36 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 589-972 of human USP36 (NP_079366) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

122.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for USP36 Antibody (OTI7G3) - Azide and BSA Free

Western Blot: USP36 Antibody (OTI7G3)Azide and BSA Free [NBP2-74806]

Western Blot: USP36 Antibody (OTI7G3)Azide and BSA Free [NBP2-74806]

Western Blot: USP36 Antibody (OTI7G3) - Azide and BSA Free [NBP2-74806] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP36 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP36.
Immunocytochemistry/ Immunofluorescence: USP36 Antibody (OTI7G3) - Azide and BSA Free [NBP2-74806]

Immunocytochemistry/ Immunofluorescence: USP36 Antibody (OTI7G3) - Azide and BSA Free [NBP2-74806]

Immunocytochemistry/Immunofluorescence: USP36 Antibody (OTI7G3) - Azide and BSA Free [NBP2-74806] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY USP36.
Immunohistochemistry: USP36 Antibody (OTI7G3) - Azide and BSA Free [NBP2-74806]

Immunohistochemistry: USP36 Antibody (OTI7G3) - Azide and BSA Free [NBP2-74806]

Immunohistochemistry: USP36 Antibody (OTI7G3) - Azide and BSA Free [NBP2-74806] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-USP36 mouse monoclonal antibody.
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Applications for USP36 Antibody (OTI7G3) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000
Application Notes
Use in KD reported in scientific literature (PMID:34526665).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: USP36

USP36 belongs to the peptidase C19 family and is a deubiquitinating enzyme. USP36 contains the PEST motif and is polyubiquitinated. USP36 is broadly expressed. There are two named isoforms.

Long Name

Ubiquitin Specific Protease 36

Alternate Names

DUB1, KIAA1453

Gene Symbol

USP36

Additional USP36 Products

Product Documents for USP36 Antibody (OTI7G3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for USP36 Antibody (OTI7G3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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