USP4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2868

Novus Biologicals, part of Bio-Techne
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NB100-2868
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all USP4 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 225 and 275 of human Ubiquitin Specific Protease 4 using the numbering given in entry AAB72237.1 (GeneID 7375).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for USP4 Antibody

Immunoprecipitation: USP4 Antibody [NB100-2868]

Immunoprecipitation: USP4 Antibody [NB100-2868]

Immunoprecipitation: USP4 Antibody [NB100-2868] - Detection of Human USP4 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-USP4 antibody NB100-2868 used for IP at 3 mcg/mg lysate. USP4 was also immunoprecipitated by rabbit anti-USP4 antibody NB100-2869, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for USP4 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
Not Recommended for Western Blot. Use rabbit anti-USP4 Antibody NB 100-2868.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: USP4

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Long Name

Ubiquitin Specific Protease 4

Alternate Names

UNP, Unph

Entrez Gene IDs

7375 (Human)

Gene Symbol

USP4

UniProt

AAB72237.1 (Human)

Additional USP4 Products

Product Documents for USP4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for USP4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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