Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all USP4 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 925 and the C-terminus (residue 963) of human Ubiquitin Specific Protease 4 using the numbering given in entry AAB72237.1 (GeneID 7375).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for USP4 Antibody - BSA Free

Western Blot: USP4 Antibody [NB100-2869]

Western Blot: USP4 Antibody [NB100-2869]

Western Blot: USP4 Antibody [NB100-2869] - Detection of Human and Mouse USP4 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP4 antibody NB100-2869 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: USP4 Antibody [NB100-2869]

Immunoprecipitation: USP4 Antibody [NB100-2869]

Immunoprecipitation: USP4 Antibody [NB100-2869] - Detection of human USP4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP4 antibody NB100-2869 used for IP at 6 ug per reaction. USP4 was also immunoprecipitated by rabbit anti-USP4 antibody NB100-2868. For blotting immunoprecipitated USP4, NB100-2869 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for USP4 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: USP4

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Long Name

Ubiquitin Specific Protease 4

Alternate Names

UNP, Unph

Entrez Gene IDs

7375 (Human)

Gene Symbol

USP4

UniProt

AAB72237.1 (Human)

Additional USP4 Products

Product Documents for USP4 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for USP4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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