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USP46 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-35794

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-35794-100ul
NBP3-35794-20ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human USP46 (NP_073743.2).

Sequence:
KKFISRLRKENDLFDNYMQQDAHEFLNYLLNTIADILQEEKKQEKQNGKLKNGNMNEPAENNKPELTWVHEIFQGTLTNETRCLNCETVSSKDEDFLDLSV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for USP46 Antibody

USP46 Antibody

Western Blot: USP46 Antibody [NBP3-35794] -

Western Blot: USP46 Antibody [NBP3-35794] - Western blot analysis of lysates from mouse pancreas, using USP46 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
USP46 Antibody

Immunocytochemistry/ Immunofluorescence: USP46 Antibody [NBP3-35794] -

Immunocytochemistry/ Immunofluorescence: USP46 Antibody [NBP3-35794] - Immunofluorescence analysis of C6 cells using USP46 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
USP46 Antibody

Immunocytochemistry/ Immunofluorescence: USP46 Antibody [NBP3-35794] -

Immunocytochemistry/ Immunofluorescence: USP46 Antibody [NBP3-35794] - Immunofluorescence analysis of L-929 cells using USP46 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for USP46 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:100

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: USP46

Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinatedaction of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP46 belongs to a large family of cysteineproteases that function as deubiquitinating enzymes (Quesada et al., 2004 (PubMed 14715245)).(supplied by OMIM)

Long Name

Ubiquitin Specific Protease 46

Alternate Names

Deubiquitinating enzyme 46, EC 3.1.2.15, EC 3.4.19.12, FLJ11850, FLJ12552, FLJ14283, FLJ39393, ubiquitin carboxyl-terminal hydrolase 46, ubiquitin specific peptidase 46, ubiquitin specific protease 46, ubiquitin thioesterase 46, Ubiquitin thiolesterase 46, Ubiquitin-specific-processing protease 46

Gene Symbol

USP46

Additional USP46 Products

Product Documents for USP46 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for USP46 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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