USP47 Antibody (5F9)
Novus Biologicals, part of Bio-Techne | Catalog # H00055031-M01
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 5F9
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
USP47 (NP_060414, 203 a.a. ~ 301 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TEQADLINELYQGKLKDYVRCLECGYEGWRIDTYLDIPLVIRPYGSSQAFASVEEALHAFIQPEILDGPNQYFCERCKKKCDARKGLRFLHFPYLLTLQ
Reactivity Notes
Human. Other species not tested.
Specificity
USP47 - ubiquitin specific protease 47
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for USP47 Antibody (5F9)
![Western Blot: USP47 Antibody (5F9) [H00055031-M01]](https://resources.bio-techne.com/images/products/USP47-Antibody-5F9-Western-Blot-H00055031-M01-img0007.jpg "Western Blot: USP47 Antibody (5F9) [H00055031-M01]")
Western Blot: USP47 Antibody (5F9) [H00055031-M01]
Western Blot: USP47 Antibody (5F9) [H00055031-M01] - USP47 monoclonal antibody (M01), clone 5F9 Analysis of USP47 expression in HeLa.![Immunocytochemistry/ Immunofluorescence: USP47 Antibody (5F9) [H00055031-M01]](https://resources.bio-techne.com/images/products/USP47-Antibody-5F9-Immunocytochemistry-Immunofluorescence-H00055031-M01-img0005.jpg "Immunocytochemistry/ Immunofluorescence: USP47 Antibody (5F9) [H00055031-M01]")
Immunocytochemistry/ Immunofluorescence: USP47 Antibody (5F9) [H00055031-M01]
Immunocytochemistry/Immunofluorescence: USP47 Antibody (5F9) [H00055031-M01] - Analysis of monoclonal antibody to USP47 on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: USP47 Antibody (5F9) [H00055031-M01] - Detection limit for recombinant GST tagged USP47 is approximately 0.3ng/ml as a capture antibody.
Applications for USP47 Antibody (5F9)
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
1:10-1:2000
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: USP47
Long Name
Ubiquitin Specific Protease 47
Alternate Names
TRFP
Entrez Gene IDs
55031 (Human)
Gene Symbol
USP47
UniProt
Additional USP47 Products
Product Documents for USP47 Antibody (5F9)
Product Specific Notices for USP47 Antibody (5F9)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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