USP48 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83751

Novus Biologicals, part of Bio-Techne
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NBP1-83751
NBP1-83751-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all USP48 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QEKPNTTVQVPAFLQELVDRDNSKFEEWCIEMAEMRKQSVDKGKAKHEEVKELYQRLPAGAEPYEFVSLEWLQKWLDESTPTKPIDNHACLCSHDKLHPDKISIMKRISEYAADIFYSRYGGGPRLTVKALCKECVV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for USP48 Antibody

Western Blot: USP48 Antibody [NBP1-83751]

Western Blot: USP48 Antibody [NBP1-83751]

Western Blot: USP48 Antibody [NBP1-83751] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/ Immunofluorescence: USP48 Antibody [NBP1-83751]

Immunocytochemistry/ Immunofluorescence: USP48 Antibody [NBP1-83751]

Immunocytochemistry/Immunofluorescence: USP48 Antibody [NBP1-83751] - Staining of human cell line U-251 MG shows localization to nucleoplasm & mitochondria. Antibody staining is shown in green.
Immunohistochemistry: USP48 Antibody [NBP1-83751]

Immunohistochemistry: USP48 Antibody [NBP1-83751]

Immunohistochemistry: USP48 Antibody [NBP1-83751] - Staining of mouse visual cortex shows immunoreactivity in neurons and endothelial cells.
View 8 more images

Applications for USP48 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: USP48

The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific peptidase 48 (USP48) is a member of the C19 peptidase family involved in the processing of polyubiquitin precursors as well as that of ubiquitinated proteins. USP48 is involved in the activation of NF-kappa B via its interaction with RELA and TRAF2.

Long Name

Ubiquitin Specific Protease 48

Alternate Names

RAP1GA1, synUSP, USP31

Gene Symbol

USP48

Additional USP48 Products

Product Documents for USP48 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for USP48 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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