USP9x Antibody (JG35-11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75704

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JG35-11

Concentration

1 mg/ml

View all USP9x Antibodies »

Product Specifications

Immunogen

Recombinant protein within Human USP9x aa 1-118 / 2,554. (SwissProt: Q93008 Human; SwissProt: P70398 Mouse; SwissProt: D3ZC84 Rat)

Localization

Cell projection, Cytoplasm, Cytoskeleton.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

290 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for USP9x Antibody (JG35-11)

Western Blot: USP9x Antibody (JG35-11) [NBP2-75704]

Western Blot: USP9x Antibody (JG35-11) [NBP2-75704]

Western Blot: USP9x Antibody (JG35-11) [NBP2-75704] - Analysis of USP9X on different lysates using anti-USP9X antibody at 1/1,000 dilution.Positive control:Lane 1: SiHa Lane 2: A549Lane 3: 293 Lane 4: Mouse colon
Immunocytochemistry/ Immunofluorescence: USP9x Antibody (JG35-11) [NBP2-75704]

Immunocytochemistry/ Immunofluorescence: USP9x Antibody (JG35-11) [NBP2-75704]

Immunocytochemistry/Immunofluorescence: USP9x Antibody (JG35-11) [NBP2-75704] - Staining USP9X in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: USP9x Antibody (JG35-11) [NBP2-75704]

Immunohistochemistry-Paraffin: USP9x Antibody (JG35-11) [NBP2-75704]

Immunohistochemistry-Paraffin: USP9x Antibody (JG35-11) [NBP2-75704] - Analysis of paraffin-embedded mouse fallopian tube tissue using anti-USP9X antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
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Applications for USP9x Antibody (JG35-11)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: USP9x

USP9X is an ubiquitin-specific protease involved in the removal and recycling of ubiquitin from proteins degraded in proteasomes. USP9X is located on the X-chromosome but escapes X-inactivation. Defects in USP9X may cause gonadal degeneration in Turner syndrome.

Long Name

Ubiquitin-specific Protease 9, X-linked

Alternate Names

DFFRX, FAF, FAM

Gene Symbol

USP9X

Additional USP9x Products

Product Documents for USP9x Antibody (JG35-11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for USP9x Antibody (JG35-11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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