Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI2G7

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all USP9x Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 2246-2570 of human USP9X (NP_001034680) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for USP9x Antibody (OTI2G7) - Azide and BSA Free

Western Blot: USP9x Antibody (OTI2G7)Azide and BSA Free [NBP2-74822]

Western Blot: USP9x Antibody (OTI2G7)Azide and BSA Free [NBP2-74822]

Western Blot: USP9x Antibody (OTI2G7) - Azide and BSA Free [NBP2-74822] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP9x (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP9x.
Immunohistochemistry: USP9x Antibody (OTI2G7) - Azide and BSA Free [NBP2-74822]

Immunohistochemistry: USP9x Antibody (OTI2G7) - Azide and BSA Free [NBP2-74822]

Immunohistochemistry: USP9x Antibody (OTI2G7) - Azide and BSA Free [NBP2-74822] - Staining of paraffin-embedded Human liver tissue using anti-USP9x mouse monoclonal antibody.
Western Blot: USP9x Antibody (OTI2G7)Azide and BSA Free [NBP2-74822]

Western Blot: USP9x Antibody (OTI2G7)Azide and BSA Free [NBP2-74822]

Western Blot: USP9x Antibody (OTI2G7) - Azide and BSA Free [NBP2-74822] - Analysis of extracts (35ug) from 9 different cell lines by using g anti-USP9x monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Applications for USP9x Antibody (OTI2G7) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: USP9x

USP9X is an ubiquitin-specific protease involved in the removal and recycling of ubiquitin from proteins degraded in proteasomes. USP9X is located on the X-chromosome but escapes X-inactivation. Defects in USP9X may cause gonadal degeneration in Turner syndrome.

Long Name

Ubiquitin-specific Protease 9, X-linked

Alternate Names

DFFRX, FAF, FAM

Gene Symbol

USP9X

Additional USP9x Products

Product Documents for USP9x Antibody (OTI2G7) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for USP9x Antibody (OTI2G7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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