Vanilloid R1/TRPV1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-98886
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
A synthetic peptide from the 1t cytoplasmic loop of human Vanilloid R1/TRPV1 conjugated to blue carrier protein was used as the antigen.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 21515789).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Vanilloid R1/TRPV1 Antibody
Western Blot: Vanilloid R1/TRPV1 Antibody [NB100-98886]
Western Blot: Vanilloid R1/TRPV1 Antibody [NB100-98886] - The expressed and electrophysiology properties of Vanilloid R1/TRPV1 in primary cultured microglia. Representative Immunoblot bands and densitometric analysis of total protein (E) and membrane protein expression (F) of Vanilloid R1/TRPV1 in cortical primary cultured microglia with or without pretreatment of CAP (10 uM), hyperthermia (43C, 4*30 min) or LPS (1.0 ug/ml, 24 h). Data were presented as means +/- SEM, n = 3 per groups, *p < 0.05, **p < 0.01, one-way analysis of variance (ANOVA) followed by Dunnett's multiple comparison test with DMSO. Ctrl, Control; CAP, capsaicin; HT, hyperthermia. Image collected and cropped by CiteAb from the following publication (www.frontiersin.org/articles/10.3389/fncel.2019.00442/full) licensed under a CC-BY license.Immunocytochemistry/ Immunofluorescence: Vanilloid R1/TRPV1 Antibody [NB100-98886]
Immunocytochemistry/Immunofluorescence: Vanilloid R1/TRPV1 Antibody [NB100-98886] - Vanilloid R1/TRPV1 and toll-like receptor 4 (TLR4) mutually inhibited the transforming growth factor-beta1 (TGF-beta1) signaling in activated microglia. Photomicrograph immunocytochemistry indicated different co-expression of Vanilloid R1/TRPV1 (visualized in red Cy3), TLR4 (visualized in green DyLight 488), and cell nuclei (visualized in dark blue DAPI) in activated microglia. Image collected and cropped by CiteAb from the following publication (www.frontiersin.org/article/10.3389/fncel.2019.00442/full) licensed under a CC-BY license.Immunohistochemistry-Paraffin: Vanilloid R1/TRPV1 Antibody [NB100-98886]
Immunohistochemistry-Paraffin: Vanilloid R1/TRPV1 Antibody [NB100-98886] - Human hippocampus. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 umApplications for Vanilloid R1/TRPV1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:1000
Immunohistochemistry-Paraffin
1:1000
Western Blot
1:1000
Application Notes
IHC reported in scientific literature (PMID: 31680864). ICC/IF reported in scientific literature (PMID: 21515789).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Unpurified
Reconstitution
Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.
Formulation
Lyophilized from whole antisera
Preservative
No Preservative
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Vanilloid R1/TRPV1
Long Name
Vanilloid Receptor 1/Transient Receptor Potential Cation Channel 1
Alternate Names
Capsaicin Receptor, TRPV1, VanilloidR1, VR1
Entrez Gene IDs
7442 (Human)
Gene Symbol
TRPV1
UniProt
Additional Vanilloid R1/TRPV1 Products
Product Documents for Vanilloid R1/TRPV1 Antibody
Product Specific Notices for Vanilloid R1/TRPV1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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