VAP-B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89112

Novus Biologicals, part of Bio-Techne
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NBP1-89112
NBP1-89112-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all VAP-B Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VWKEAKPEDLMDSKLRCVFELPAENDKPHDVEINKIISTTASKTETPIVSKSLSSSLDDTEVKKVMEECKRLQGEVQRLREENKQFKEEDGLRMRKTVQSNSPISALAPTGK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VAP-B Antibody

Western Blot: VAP-B Antibody [NBP1-89112]

Western Blot: VAP-B Antibody [NBP1-89112]

Western Blot: VAP-B Antibody [NBP1-89112] - Analysis in human cell lines Caco-2 and HEK293 using anti-VAPB antibody. Corresponding VAPB RNA-seq data are presented for the same cell lines. Loading control: anti-COX4I1.
Immunocytochemistry/ Immunofluorescence: VAP-B Antibody [NBP1-89112]

Immunocytochemistry/ Immunofluorescence: VAP-B Antibody [NBP1-89112]

Immunocytochemistry/Immunofluorescence: VAP-B Antibody [NBP1-89112] - Staining of human cell line U-2 OS shows positivity in endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: VAP-B Antibody [NBP1-89112]

Immunohistochemistry-Paraffin: VAP-B Antibody [NBP1-89112]

Immunohistochemistry-Paraffin: VAP-B Antibody [NBP1-89112] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
View 4 more images

Applications for VAP-B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
Use in Immunoprecipitation reported in scientific literature (PMID:32752233). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization, Use PFA/Triton X-100. VAP-B antibody validated for ICC/IF from a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NBP1-89112 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VAP-B

May play a role in vesicle trafficking. Homodimer, and heterodimer with VAPA. Interacts with VAMP1 and VAMP2.

Long Name

VAMP [Vesicle-associated Membrane Protein]-associated Protein B and C

Alternate Names

ALS8, VAMP-B, VAMP-C, VAP-C, VAPB

Gene Symbol

VAPB

Additional VAP-B Products

Product Documents for VAP-B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for VAP-B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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