VASA Antibody (2F9H5) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51586

Novus Biologicals, part of Bio-Techne
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NBP1-51586
NBP1-51586-0.025ml
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2F9H5

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all VASA Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human VASA expressed in E. Coli.

Marker

Primordial Germ Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VASA Antibody (2F9H5) - BSA Free

Western Blot: VASA Antibody (2F9H5)BSA Free [NBP1-51586]

Western Blot: VASA Antibody (2F9H5)BSA Free [NBP1-51586]

Western Blot: VASA Antibody (2F9H5) [NBP1-51586] - Analysis using DDX4 mouse mAb against DDX4-hIgGFc transfected HEK293 cell lysate.
Immunocytochemistry/ Immunofluorescence: VASA Antibody (2F9H5) - BSA Free [NBP1-51586]

Immunocytochemistry/ Immunofluorescence: VASA Antibody (2F9H5) - BSA Free [NBP1-51586]

Immunocytochemistry/Immunofluorescence: VASA Antibody (2F9H5) [NBP1-51586] - Analysis of MSCs(left) and NTERA-2 (right) cells using DDX4 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
Immunohistochemistry-Paraffin: VASA Antibody (2F9H5) - BSA Free [NBP1-51586]

Immunohistochemistry-Paraffin: VASA Antibody (2F9H5) - BSA Free [NBP1-51586]

Immunohistochemistry-Paraffin: VASA Antibody (2F9H5) [NBP1-51586] - Analysis of human lung cancer (A) and rectal cancer (B), showing cytoplasmic localization using DDX4 mouse mAb with DAB staining.
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Applications for VASA Antibody (2F9H5) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VASA

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development.

Long Name

DEAD [Asp-Glu-Ala-Asp] Box Polypeptide 41

Alternate Names

DDX4

Entrez Gene IDs

54514 (Human)

Gene Symbol

DDX4

UniProt

Q9NQI0

Additional VASA Products

Product Documents for VASA Antibody (2F9H5) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for VASA Antibody (2F9H5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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