VASP Antibody (GT238)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13619

Novus Biologicals, part of Bio-Techne
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NBP3-13619
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # GT238

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all VASP Antibodies »

Product Summary for VASP Antibody (GT238)

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human VASP. The exact sequence is proprietary.

Reactivity Notes

Mouse (84%), Rat (84%), Dog (88%), Bovine (88%)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

Centrifuge briefly prior to opening.

Scientific Data Images for VASP Antibody (GT238)

Western Blot: VASP Antibody (GT238) [NBP3-13619]

Western Blot: VASP Antibody (GT238) [NBP3-13619]

Western Blot: VASP Antibody (GT238) [NBP3-13619] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with VASP antibody [GT238] (NBP3-13619) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: VASP Antibody (GT238) [NBP3-13619]

Immunocytochemistry/ Immunofluorescence: VASP Antibody (GT238) [NBP3-13619]

Immunocytochemistry/Immunofluorescence: VASP Antibody (GT238) [NBP3-13619] - VASP antibody [GT238] detects VASP protein at focal adhesion by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VASP stained by VASP antibody [GT238] (NBP3-13619) diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: VASP Antibody (GT238) [NBP3-13619]

Immunohistochemistry-Paraffin: VASP Antibody (GT238) [NBP3-13619]

Immunohistochemistry-Paraffin: VASP Antibody (GT238) [NBP3-13619] - VASP antibody [GT238] detects VASP protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. Green: VASP stained by VASP antibody [GT238] (NBP3-13619) diluted at 1:250. Red: beta Actin, a cytoskeleton marker, stained by beta Actin antibody (NBP2-15569) diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for VASP Antibody (GT238)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VASP

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. VASP shares a limited degree of homology with the amino terminus of WASP, which is frequently mutated in Wiskott-Aldrich syndrome (WAS) patients. An established substrate of cAMP and cGMP dependent kinases, VASP is phosphorylated on a regulatory serine residue 157 and localizes to focal adhesions, microfilaments and highly active regions of the plasma membrane. VASP is highly expressed in human platelets and, like WASP, may play a role in cytoskeletal organization.

Long Name

Vasodilator-stimulated Phosphoprotein

Alternate Names

vasodilator-stimulated phosphoprotein

Gene Symbol

VASP

Additional VASP Products

Product Documents for VASP Antibody (GT238)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for VASP Antibody (GT238)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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