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VASP [p Ser238] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-82254

Novus Biologicals, part of Bio-Techne
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NB100-82254
NB100-82254-0.025ml

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Summary for VASP [p Ser238] Antibody

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human VASP around the phosphorylation site of serine 238 (K-V-SP-K-Q).

Modification

p Ser238

Specificity

Detects endogenous levels of VASP only when phosphorylated at serine 238.

Marker

Focal Adhesion Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VASP [p Ser238] Antibody

Western Blot: VASP [p Ser238] Antibody [NB100-82254]

Western Blot: VASP [p Ser238] Antibody [NB100-82254]

Western Blot: VASP [p Ser238] Antibody [NB100-82254] - Analysis of extracts from serum-treated 293 and PMA-treated C6 cells using VASP (Phospho-Ser239) Antibody

Immunohistochemistry-Paraffin: VASP [p Ser238] Antibody [NB100-82254] -

Immunohistochemistry-Paraffin: VASP [p Ser238] Antibody [NB100-82254] - Immunohistochemical analysis of paraffin-embedded human tonsil carcinoma tissue, using NB100-82254 (left) or the same antibody preincubated with blocking peptide(right).

Applications for VASP [p Ser238] Antibody

Application
Recommended Usage

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VASP

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.

Long Name

Vasodilator-stimulated Phosphoprotein

Alternate Names

vasodilator-stimulated phosphoprotein

Entrez Gene IDs

7408 (Human); 22323 (Mouse); 361517 (Rat)

Gene Symbol

VASP

UniProt

Additional VASP Products

Product Documents for VASP [p Ser238] Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VASP [p Ser238] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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