VASP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85792

Novus Biologicals, part of Bio-Techne
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NBP1-85792
NBP1-85792-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all VASP Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PKAESGRSGGGGLMEEMNAMLARRRKATQVGEKTPKDESANQEEPEARVPAQSESVRRPWEKNSTTLPRMKSSSSVTTSETQPCTPSSSDYSDLQRVKQELLEEVKKELQKVK

Reactivity Notes

Reactivity reported in scientific literature (PMID: 22361696)

Marker

Focal Adhesion Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VASP Antibody

Immunohistochemistry-Paraffin: VASP Antibody [NBP1-85792]

Immunohistochemistry-Paraffin: VASP Antibody [NBP1-85792]

Immunohistochemistry-Paraffin: VASP Antibody [NBP1-85792] - Analysis in human lymph node and skeletal muscle tissues using NBP1-85792 antibody. Corresponding VASP RNA-seq data are presented for the same tissues.
Western Blot: VASP Antibody [NBP1-85792]

Western Blot: VASP Antibody [NBP1-85792]

Western Blot: VASP Antibody [NBP1-85792] - Analysis in human cell lines U-251MG and HEK293 using Anti-VASP antibody. Corresponding VASP RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Immunocytochemistry/ Immunofluorescence: VASP Antibody [NBP1-85792]

Immunocytochemistry/ Immunofluorescence: VASP Antibody [NBP1-85792]

Immunocytochemistry/Immunofluorescence: VASP Antibody [NBP1-85792] - Staining of human cell line A-431 shows localization to plasma membrane, cytosol & focal adhesion sites. Antibody staining is shown in green.
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Applications for VASP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VASP

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. VASP shares a limited degree of homology with the amino terminus of WASP, which is frequently mutated in Wiskott-Aldrich syndrome (WAS) patients. An established substrate of cAMP and cGMP dependent kinases, VASP is phosphorylated on a regulatory serine residue 157 and localizes to focal adhesions, microfilaments and highly active regions of the plasma membrane. VASP is highly expressed in human platelets and, like WASP, may play a role in cytoskeletal organization.

Long Name

Vasodilator-stimulated Phosphoprotein

Alternate Names

vasodilator-stimulated phosphoprotein

Gene Symbol

VASP

Additional VASP Products

Product Documents for VASP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for VASP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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