VASP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42515

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-42515
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all VASP Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RKVSKQEEASGGPT corresponding to internal region according to NP_003361.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for VASP Antibody

Western Blot: VASP Antibody [NBP2-42515]

Western Blot: VASP Antibody [NBP2-42515]

Western Blot: VASP Antibody [NBP2-42515] - (1ug/ml) staining of Mouse Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: VASP Antibody [NBP2-42515]

Western Blot: VASP Antibody [NBP2-42515]

Western Blot: VASP Antibody [NBP2-42515] - (0.01ug/ml) staining of Human Platelets lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for VASP Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:128000

Western Blot

1-3ug/ml
Application Notes
Approx 50kDa band observed in Human Platelets lysates (calculated MW of 39.8kDa according to NP_003361.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Reinhard et al, EMBO J. 1992 Jun;11(6):2063-70. PMID: 1318192). Recommended concentration: 0.01-0.03ug/ml. Approx 40kDa band observed in Mouse Thymus lysates (calculated MW of 39.7kDa according to NP_033525.2).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VASP

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. VASP shares a limited degree of homology with the amino terminus of WASP, which is frequently mutated in Wiskott-Aldrich syndrome (WAS) patients. An established substrate of cAMP and cGMP dependent kinases, VASP is phosphorylated on a regulatory serine residue 157 and localizes to focal adhesions, microfilaments and highly active regions of the plasma membrane. VASP is highly expressed in human platelets and, like WASP, may play a role in cytoskeletal organization.

Long Name

Vasodilator-stimulated Phosphoprotein

Alternate Names

vasodilator-stimulated phosphoprotein

Entrez Gene IDs

7408 (Human)

Gene Symbol

VASP

Additional VASP Products

Product Documents for VASP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for VASP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.