VAV2 Antibody (Polyclonal)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-30791
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG Clone # Polyclonal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Sheep were immunized with a his tagged fusion protein VAV2 corresponding to amino acid sequence 287-578 and having a molecular weight of 100kD
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for VAV2 Antibody (Polyclonal)
Western Blot: VAV2 Antibody (Polyclonal) [NBP3-30791] -
Western Blot: VAV2 Antibody (Polyclonal) [NBP3-30791] - Legend: HEK293 cells were transfected with either emply expression vector (pSR␣ ) or pSRa-Vav2 driving expression of human Vav2. Indicated antibodies (SHD or DPH) were used to immunoprecipitate Vav2 from 300ug protein lysates. Immunoprecipitated activity was identified by immunoblot of complexes using either DPH (upper gel) or SHD (lower gel) antisera at (1:10000). Immunoprecipitation of Endogenous/Exogenous Vav2 from HEK 283 (75 ug lysate per lane). Top gel - i.p. Ab: DPH or SHD preimmune serum (2.5 ul/300ug lysate). DPH or SHD alpha Vav2 antiserum (2.5 ul/300ug lysate). Western Blot Ab: DPH anti Vav2 1:10000. Bottom Gel - i.p. Ab DPH or SHD preimmune sera (2.5 ul/300ug lysate). DPH or SHD anti Vav2 antisera (2.5ul/300ug lysate). Wrester blot Ab - SHD anti Vav2 antibody 1:10000.Applications for VAV2 Antibody (Polyclonal)
Application
Recommended Usage
Western Blot
: 1:2500 -1:5000
Application Notes
.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Ammonium sulfate precipitation
Formulation
Phosphate buffered saline
Preservative
0.08% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: VAV2
Alternate Names
guanine nucleotide exchange factor VAV2, oncogene VAV2, Protein vav-2, vav 2 guanine nucleotide exchange factor, vav 2 oncogene, VAV-2
Gene Symbol
VAV2
Additional VAV2 Products
Product Specific Notices for VAV2 Antibody (Polyclonal)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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