Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

ELISA, Immunoblotting, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6G9

Format

Azide and BSA Free

Concentration

1 mg/ml

View all VCAM-1/CD106 Antibodies »

Product Summary for VCAM-1/CD106 Antibody (6G9) - Azide and BSA Free

Immunogen

Purified recombinant fragment of human VCAM-1 expressed in E. coli.

Localization

Membrane; Single-pass type I membrane protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VCAM-1/CD106 Antibody (6G9) - Azide and BSA Free

Western Blot: VCAM-1/CD106 Antibody (6G9)Azide and BSA Free [NBP2-81005]

Western Blot: VCAM-1/CD106 Antibody (6G9)Azide and BSA Free [NBP2-81005]

Western Blot: VCAM-1/CD106 Antibody (6G9) - Azide and BSA Free [NBP2-81005] - Using VCAM1 mouse mAb against (1) HUVEC and (2) EC cell lysate. Image from the standard format of this antibody.
Immunohistochemistry: VCAM-1/CD106 Antibody (6G9) - Azide and BSA Free [NBP2-81005]

Immunohistochemistry: VCAM-1/CD106 Antibody (6G9) - Azide and BSA Free [NBP2-81005]

Immunohistochemistry: VCAM-1/CD106 Antibody (6G9) - Azide and BSA Free [NBP2-81005] - Human placenta tissues using VCAM1 mouse mAb. Image from the standard format of this antibody.
Immunohistochemistry: VCAM-1/CD106 Antibody (6G9) - Azide and BSA Free [NBP2-81005]

Immunohistochemistry: VCAM-1/CD106 Antibody (6G9) - Azide and BSA Free [NBP2-81005]

Immunohistochemistry: VCAM-1/CD106 Antibody (6G9) - Azide and BSA Free [NBP2-81005] - Breast carcinoma, cytoplasmic staining. Image from verified customer review. Image from the standard format of this antibody.
View 2 more images

Applications for VCAM-1/CD106 Antibody (6G9) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunoblotting

reported in scientific literature (PMID 28569748)

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Immunoprecipitation

reported in scientific literature (PMID 28569748)

Simple Western

1:1000

Western Blot

1:500-1:2000
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VCAM-1/CD106

VCAM1 is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. Tissue specificity: Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue.

Long Name

Vascular Cell Adhesion Molecule 1

Alternate Names

CD106, VCAM1

Gene Symbol

VCAM1

Additional VCAM-1/CD106 Products

Product Documents for VCAM-1/CD106 Antibody (6G9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for VCAM-1/CD106 Antibody (6G9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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