VCAM-1/CD106 Antibody (B-K9) [PE/Cy5.5]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47864PECY55
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Rat
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence
Label
PE/Cy5.5 (Excitation = 488 nm, Emission = 695 nm)
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # B-K9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Activated human umbilical vein endothelial cells (HUVEC)
Reactivity Notes
Use in Rat reported in scientific literature (PMID:32152303).
Localization
Cell surface
Specificity
Recognizes a protein of 110kDa, identified as CD106 (also known as vascular cell adhesion molecule-1 (VCAM-1) and INCAM-100). CD106 is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on un-stimulated endothelial cells. It is also present on follicular and inter-follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. CD106 serves as a ligand for leukocyte integrin (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in various immunological and inflammatory responses. This monoclonal antibody inhibits the binding of leukocytes to VCAM-1 on stimulated endothelial cells.
Marker
Activated Endothelial Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Applications for VCAM-1/CD106 Antibody (B-K9) [PE/Cy5.5]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: VCAM-1/CD106
Long Name
Vascular Cell Adhesion Molecule 1
Alternate Names
CD106, VCAM1
Gene Symbol
VCAM1
Additional VCAM-1/CD106 Products
Product Documents for VCAM-1/CD106 Antibody (B-K9) [PE/Cy5.5]
Product Specific Notices for VCAM-1/CD106 Antibody (B-K9) [PE/Cy5.5]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...