VCAM-1/CD106 Antibody (MR106) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43368

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Rat

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # MR106

Format

BSA Free

Concentration

0.5 mg/ml

View all VCAM-1/CD106 Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was CD106.

Reactivity Notes

This antibody is reactive to Rat.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for VCAM-1/CD106 Antibody (MR106) - BSA Free

Western Blot: VCAM-1/CD106 Antibody (MR106)BSA Free [NBP1-43368]

Western Blot: VCAM-1/CD106 Antibody (MR106)BSA Free [NBP1-43368]

Western Blot: VCAM-1/CD106 Antibody (MR106) [NBP1-43368] - Western blot analyses of protein expressions of glutathione peroxidase-1 (GPx1), vascular cell adhesion molecule 1 (VCAM-1), inducible nitric oxide (iNOS), nuclear factor (NF-kappaB), matrix metalloproteinase-9 (MMP-9), and surfactant protein A and D (SP-A and SP-D) in lung (n = 3; * and # signify P < 0.05 and P < 0.001, respectively) Image collected and cropped by CiteAb from the following publication (https://bmcpulmmed.biomedcentral.com/articles/10.1186/s12890-017-0448-9), licensed under a CC-BY license.
Flow Cytometry: VCAM-1/CD106 Antibody (MR106) - BSA Free [NBP1-43368]

Flow Cytometry: VCAM-1/CD106 Antibody (MR106) - BSA Free [NBP1-43368]

Flow Cytometry: VCAM-1/CD106 Antibody (MR106) [NBP1-43368] - Analysis using the Biotin conjugate of NBP1-43368. Staining of rat bone marrow cells with 0.125 ug of Mouse IgG1 kappa Isotype Control Biotin (left) or 0.125 ug of Anti-Rat CD106 (VCAM-1) Biotin (right) followed by Streptavidin PE.
Flow Cytometry: VCAM-1/CD106 Antibody (MR106) - BSA Free [NBP1-43368]

Flow Cytometry: VCAM-1/CD106 Antibody (MR106) - BSA Free [NBP1-43368]

Flow Cytometry: VCAM-1/CD106 Antibody (MR106) [NBP1-43368] - Staining of rat bone marrow cells with 0.25 ug of Mouse IgG1 kappa Isotype Control Purified (open histogram) or 0.25 ug of Anti-Rat CD106 (VCAM-1) Purified (filled histogram) followed by Anti-Mouse IgG Biotin and Streptavidin PE . Cells in the large scatt
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Applications for VCAM-1/CD106 Antibody (MR106) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
This MR106 antibody has been tested by flow cytometric analysis of rat bone marrow cells. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: VCAM-1/CD106

The MR106 monoclonal antibody reacts with rat CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and further upregulate VCAM-1 upon cytokine stimulation. CD106 binds to integrin alpha4beta1 (VLA-4, CD49d/CD29) and Integrin alpha4beta7 (LPAM-1) and these interactions in the bone marrow and thymus are important for early lymphocyte and myeloid development. Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response.

Long Name

Vascular Cell Adhesion Molecule 1

Alternate Names

CD106, VCAM1

Gene Symbol

VCAM1

Additional VCAM-1/CD106 Products

Product Documents for VCAM-1/CD106 Antibody (MR106) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for VCAM-1/CD106 Antibody (MR106) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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