VCAM-1/CD106 Antibody (VCAM1/843)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44615

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # VCAM1/843

Concentration

0.2 mg/ml

View all VCAM-1/CD106 Antibodies »

Product Summary for VCAM-1/CD106 Antibody (VCAM1/843)

Immunogen

Recombinant human VCAM-1/CD106 protein (Uniprot: P19320)

Localization

Cell surface

Marker

Activated Endothelial Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11509)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for VCAM-1/CD106 Antibody (VCAM1/843)

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (VCAM1/843) [NBP2-44615]

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (VCAM1/843) [NBP2-44615]

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (VCAM1/843) [NBP2-44615] - Human Tonsil stained with CD106 Monoclonal Antibody (VCAM1/843).

Applications for VCAM-1/CD106 Antibody (VCAM1/843)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: VCAM-1/CD106

CD106 is a 110 kD glycosylphosphatidylinositol (GPI)-linked transmembrane protein, also known as VCAM-1 and INCAM-110. It is constitutively expressed on bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells, as well as some lymphocytes. CD106 expression can be upregulated on endothelial cells by inflammatory cytokines. CD106 is involved in adhesion and acts as a counter-receptor for VLA-4 ( alpha4/ beta1 integrin) and LPAM-1 ( alpha4/ beta7 integrin). The 429 antibody has been reported to partially block VCAM-1-mediated binding.

Long Name

Vascular Cell Adhesion Molecule 1

Alternate Names

CD106, VCAM1

Gene Symbol

VCAM1

UniProt

P19320

Additional VCAM-1/CD106 Products

Product Documents for VCAM-1/CD106 Antibody (VCAM1/843)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for VCAM-1/CD106 Antibody (VCAM1/843)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.