VDAC2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-871

Novus Biologicals, part of Bio-Techne
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NB300-871
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all VDAC2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-GHKVGLALELEA corresponding to C-Terminus according to NP_003366.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

31.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VDAC2 Antibody

Western Blot: VDAC2 Antibody [NB300-871]

Western Blot: VDAC2 Antibody [NB300-871]

Western Blot: VDAC2 Antibody [NB300-871] - Staining (1ug/ml) of Human Cerebellum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: VDAC2 Antibody [NB300-871]

Immunohistochemistry-Paraffin: VDAC2 Antibody [NB300-871]

Immunohistochemistry-Paraffin: VDAC2 Antibody [NB300-871] - Staining (6ug/ml) of paraffin embedded Rat Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-Staining.
Immunohistochemistry-Paraffin: VDAC2 Antibody [NB300-871]

Immunohistochemistry-Paraffin: VDAC2 Antibody [NB300-871]

Immunohistochemistry-Paraffin: VDAC2 Antibody [NB300-871] - Negative Control showing staining of paraffin embedded Rat Liver, with no primary antibody.

Applications for VDAC2 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 - 6 ug/ml

Peptide ELISA

Detection limit 1:2000

Western Blot

0.3 - 1 ug/mL
Application Notes
WB: Approx 35 kDa band observed in Human Cerebellum lysates and in preliminary testing of CaCo-2 cell lysate (calculated MW of 31.6kDa according to Human NP_003366.2)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VDAC2

Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. Interacts with hexokinases. It is present in the outer mitochondrial membrane. There are 4 isoforms as result of alternative splicing. Expressed in all tissues examined. Consists mainly of membrane spanning sided beta sheets. Belongs to the eukaryotic mitochondrial porin family.

Alternate Names

FLJ23841, hVDAC2, Outer mitochondrial membrane protein porin 2, POR, VDAC-2, voltage-dependent anion channel 2, voltage-dependent anion-selective channel protein 2

Entrez Gene IDs

7417 (Human)

Gene Symbol

VDAC2

UniProt

P45880 (Human)

Additional VDAC2 Products

Product Documents for VDAC2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for VDAC2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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