VDR/NR1I1/Vitamin D Receptor Antibody (2F4)

Novus Biologicals, part of Bio-Techne | Catalog # H00007421-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA

Cited:

IF/IHC, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2F4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all VDR/NR1I1 Antibodies »

Product Specifications

Immunogen

VDR (AAH60832, 1 a.a. ~ 427 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEAMAASTSLPDPGDFDRNVPRICGVCGDRATGFHFNAMTCEGCKGFFRRSMKRKALFTCPFNGDCRITKDNRRHCQACRLKRCVDIGMMKEFILTDEEVQRKREMILKRKEEEALKDSLRPKLSEEQQRIIAILLDAHHKTYDPTYSDFCQFRPPVRVNDGGGSHPSRPNSRHTPSFSGDSSSSCSDHCITSSDMMDSSSFSNLDLSEEDSDDPSVTLELSQLSMLPHLADLVSYSIQKVIGFAKMIPGFRDLTSEDQIVLLKSSAIEVIMLRSNESFTMDDMSWTCGNQDYKYRVSDVTKAGHSLELIEPLIKFQVGLKKLNLHEEEHVLLMAICIVSPDRPGVQDAALIEAIQDRLSNTLQTYIRCRHPPPGSHLLYAKMIQKLADLRSLNEEHSKQYRCLSFQPECSMKLTPLVLEVFGNEIS

Specificity

VDR (2F4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for VDR/NR1I1/Vitamin D Receptor Antibody (2F4)

Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (2F4) [H00007421-M02]

Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (2F4) [H00007421-M02]

Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (2F4) [H00007421-M02] - Analysis of monoclonal antibody to VDR on formalin-fixed paraffin-embedded human lung. Antibody concentration 3 ug/ml.
Sandwich ELISA: VDR/NR1I1/Vitamin D Receptor Antibody (2F4) [H00007421-M02] - Detection limit for recombinant GST tagged VDR is approximately 1ng/ml as a capture antibody.

Applications for VDR/NR1I1/Vitamin D Receptor Antibody (2F4)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recombinant protein on ELISA. It has been used for IHC-P. Use in Immunohistochemistry reported in scientific literature (PMID: 30574409).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: VDR/NR1I1

This gene encodes the nuclear hormone receptor for vitamin D3. This receptor also functions as a receptor for the secondary bile acid lithocholic acid. The receptor belongs to the family of trans-acting transcriptional regulatory factors and shows sequence similarity to the steroid and thyroid hormone receptors. Downstream targets of this nuclear hormone receptor are principally involved in mineral metabolism though the receptor regulates a variety of other metabolic pathways, such as those involved in the immune response and cancer. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternative splicing results in multiple transcript variants encoding the same protein.

Long Name

Vitamin D [1,25-Dihydroxyvitamin D3] Receptor

Alternate Names

NR1I1

Entrez Gene IDs

7421 (Human)

Gene Symbol

VDR

OMIM

166710 (Human)

UniProt

AAH60832 (Human)

Additional VDR/NR1I1 Products

Product Documents for VDR/NR1I1/Vitamin D Receptor Antibody (2F4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for VDR/NR1I1/Vitamin D Receptor Antibody (2F4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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