VE-Cadherin Antibody (16B1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00342

Novus Biologicals, part of Bio-Techne
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NBP2-00342-100ug
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 16B1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all VE-Cadherin Antibodies »

Product Summary for VE-Cadherin Antibody (16B1)

Immunogen

The immunogen for this antibody is VE Cadherin.

Marker

Endothelial Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for VE-Cadherin Antibody (16B1)

Western Blot: VE-Cadherin Antibody (16B1) [NBP2-00342]

Western Blot: VE-Cadherin Antibody (16B1) [NBP2-00342]

Western Blot: VE-Cadherin Antibody (16B1) [NBP2-00342] - EDTA-passaged (left) and Trypsin-passaged (right) Human Umbilical Vein Endothelial Cells (HUVEC) were resolved by SDS -PAGE and immunoblotted with 2 ug/mL of Anti-Human CD144 (VE-Cadherin) Purified and revealed with Anti-Rat IgG HRP.
Flow Cytometry: VE-Cadherin Antibody (16B1) [NBP2-00342]

Flow Cytometry: VE-Cadherin Antibody (16B1) [NBP2-00342]

Flow Cytometry: VE-Cadherin Antibody (16B1) [NBP2-00342] - Analysis using the Biotin conjugate of NBP2-00342. Staining of Human Umbilical Vein Endothelial Cells (HUVEC) with 0.25 ug of Mouse IgG1 kappa Isotype Control Biotin (open histogram) or 0.125 ug of Anti-Human CD144 (VE-Cadherin) Biotin (filled histogram)
Flow Cytometry: VE-Cadherin Antibody (16B1) [NBP2-00342]

Flow Cytometry: VE-Cadherin Antibody (16B1) [NBP2-00342]

Flow Cytometry: VE-Cadherin Antibody (16B1) [NBP2-00342] - Analysis using the Allophycocyanin conjugate of NBP2-00342. Staining of Human Umbilical Vein Endothelial Cells.
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Applications for VE-Cadherin Antibody (16B1)

Application
Recommended Usage

Western Blot

1:100-1:2000
Application Notes
This 16B1 antibody has been tested by immunoblotting Human Umbilical Vein Endothelial Cell (HUVEC) lysates. This 16B1 antibody has also been reported for use in flow cytometric analysis and immunocytochemistry.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: VE-Cadherin

The 16B1 antibody reacts with human CD144, also known as VE-cadherin and cadherin-5. The cadherin family of receptors, which are calciumdependent adhesion molecules, is known to be involved in homophilic cell interactions. VE-cadherin, which is 140 kDa, is localized at the intercellular boundaries of endothelial cells in blood and lymphatic vessels in several tissues. It is thought to play a role in vascular permeability and remodeling.

Long Name

Vascular Endothelium Cadherin

Alternate Names

Cadherin-5, CD144, CDH5, VECadherin

Gene Symbol

CDH5

Additional VE-Cadherin Products

Product Documents for VE-Cadherin Antibody (16B1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for VE-Cadherin Antibody (16B1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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