VE-Cadherin Antibody (3D5C7) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37420

Novus Biologicals, part of Bio-Techne
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NBP2-37420
NBP2-37420-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

CyTOF-ready, ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3D5C7

Format

BSA Free

Concentration

1 mg/ml

View all VE-Cadherin Antibodies »

Product Summary for VE-Cadherin Antibody (3D5C7) - BSA Free

Immunogen

Purified recombinant fragment of human VE-Cadherin (AA: 29-223) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

87.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VE-Cadherin Antibody (3D5C7) - BSA Free

Western Blot: VE-Cadherin Antibody (3D5C7)BSA Free [NBP2-37420]

Western Blot: VE-Cadherin Antibody (3D5C7)BSA Free [NBP2-37420]

Western Blot: VE-Cadherin Antibody (3D5C7) [NBP2-37420] - Western blot analysis using CDH5 mouse mAb against MCF-7 (1), A549 (2), HUVE-12 (3) cell lysate, and rat lung (4) tissue lysate.
Flow Cytometry: VE-Cadherin Antibody (3D5C7) - BSA Free [NBP2-37420]

Flow Cytometry: VE-Cadherin Antibody (3D5C7) - BSA Free [NBP2-37420]

Flow Cytometry: VE-Cadherin Antibody (3D5C7) [NBP2-37420] - Flow cytometric analysis of Jurkat cells using CDH5 mouse mAb (green) and negative control (purple).
Western Blot: VE-Cadherin Antibody (3D5C7)BSA Free [NBP2-37420]

Western Blot: VE-Cadherin Antibody (3D5C7)BSA Free [NBP2-37420]

Western Blot: VE-Cadherin Antibody (3D5C7) [NBP2-37420] - Western blot analysis using CDH5 mAb against human CDH5 recombinant protein. (Expected MW is 47.6 kDa)
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Applications for VE-Cadherin Antibody (3D5C7) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 1 using NBP2-37420 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VE-Cadherin

VE-cadherin (cadherin-5) is an endothelial specific adhesion molecule that is essential for the maintenance of endothelial barrier function and angiogenesis. VE-cadherin is linked to the actin cytoskeleton through a number of adaptor proteins including a-, â-, and ã-catenin. Cytoskeletal dynamics and phosphorylation regulate VE-cadherin mediated cell-cell adhesion. The cytosolic C-terminal Src kinase (Csk) binds via its SH2 domain to the phosphorylated tyrosine 658 site of VE-cadherin. This interaction regulates density dependent cell proliferation. Phosphorylation of tyrosine 658 has also been shown to lead to the uncoupling of p120-catenin from the cytoplasmic tail of VE-cadherin. This phosphorylation event is sufficient to maintain cells in a mesenchymal state during the cell invasion phase of angiogenesis.

Long Name

Vascular Endothelium Cadherin

Alternate Names

Cadherin-5, CD144, CDH5, VECadherin

Gene Symbol

CDH5

Additional VE-Cadherin Products

Product Documents for VE-Cadherin Antibody (3D5C7) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for VE-Cadherin Antibody (3D5C7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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